HPLC测定安神补心丸中五味子醇甲、五味子酯甲和五味子甲素的含量  

Determination of schizandrol,schisanterin and deoxyschizandrin in Anshen Buxin Pills by HPLC

作　　者：宋九华[1] 杨孝容[1] 

机构地区：[1]乐山师范学院化学与生命科学系,四川乐山614004

出　　处：《中成药》2008年第3期380-383,共4页Chinese Traditional Patent Medicine

基　　金：乐山市科技局重点科技计划项目(06SZD104)

摘　　要：目的：建立安神补心丸（丹参、五味子、石昌蒲等）中五味子醇甲、五味子酯甲和五味子甲素含量的HPLC测定方法。方法：样品用超声提取，过滤，取续滤液，采用RP—HPLC法直接测定，用十八烷基键合相，甲醇-水-冰醋酸（65：35：0．1）为流动相，流速为1mL／min，检测波长为220nm。结果：五味子醇甲、五味子酯甲，五味子甲素分别在0．0012～0．5000μg（r=1．0000）、0．0014～0．5760μg（r=0．9995）和0．0021～0．8230μg（r=0．9998）范围内，质量与峰面积具有良好线性关系。平均加样回收率分别为99．51％．99．40％和99．06％，平均RSD（n=5）分别为1．48％、1．27％和1．38％。方法用于安神补心丸中五味子醇甲、五味子酯甲和五味子甲素的含量测定，RSD（n=5）分别为1．19％～1．53％、1．09％～1．52％和1．36％-1．72％。结论：方法具有灵敏度高，准确，简便，稳定快速的特点，能用于安神补心丸中五味子醇甲、五味子酯甲、五味子甲素的含量测定。AIM： To develop a HPLC method for determing the contents of schizandrol, schisantherin and deoxyschizandrin in Anshen Buxin Pills（Radix et Rhizoma Salviae miltiorrhizae, Fructus Schisandrae chinensis, Rizoma Acori tatarinowii, etc. ）. METHODS： The samples were extracted with methanol through supersonic wave and then filtered. The determination was performed by HPLC on Shim-pack VP-ODS C18 column using methanol-wateracetic acid glacial（65： 35：0.1 ） as a mobile phase, the flow rate was 1.0 mL/min, the detection wavelength was at 220 nm. RESULTS： The linear ranges of schizandrol, schisantherin and deoxyschizandrin were 0. 001 2- 0. 050 0 μg（ r = 1. 000 0 ） ,0. 001 4-0. 057 6μg（ r = 0. 999 5 ） and 0. 002 1-0. 060 8 μg（ r = 0. 999 8 ）, respectively. The average recoveries of three components were 99. 51%, 99. 40% and 99. 06%, respectively. CONCLUSION：The method is accurate, quick, sensitive and can be used for measurement of schizandrol, schisantherin and deoxyschizandrin in Anshen Buxin Pills.

关 键 词：HPLC 安神补心丸 五味子醇甲 五味子酯甲 五味子甲素 

分 类 号：R927.2[医药卫生—药学]