ELISA法检测犬腹泻粪样中的犬冠状病毒  

Detection of Canine Coronavirus in Dog Feces by ELISA

作　　者：张伯强[1] 陆承平[1] 陈怀青[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《中国兽医学报》1997年第5期437-439,共3页Chinese Journal of Veterinary Science

摘　　要：用ＦＥ细胞增殖犬冠状病毒（ＣＣＶ）参考株，分别免疫家兔和ＢＡＬＢ／ｃ小鼠制备ＣＣＶ多抗和单抗，建立了夹心ＥＬＩＳＡ及Ｄｏｔ－ＥＬＩＳＡ诊断方法。在检测的８４例犬腹泻粪样中，多抗、单抗夹心法显示ＣＣＶ阳性１６例，Ｄｏｔ－ＥＬＩＳＡ阳性１３例，后１３例包括在前１６例中。从８４例腹泻犬粪样中随机取３８例作ＣＣＶ、犬细小病毒（ＣＰＶ）双项检测，ＣＣＶ阳性１６例，ＣＰＶ阳性６例，ＣＣＶ、ＣＰＶ混合感染４例。结果显示，在南京地区流行的犬腹泻中，ＣＣＶ感染比例有超过ＣＰＶ的趋势。Polyclonal antibody (PAb) and monoclonal antibody (MAb) against canine coronavirus (CCV) were prepared by immunizing rabbits or BALB/c mice with purified CCV and used in establishing sandwich ELISA and Dot ELISA to detect CCV in fecal samples. After the cut off value in sandwich ELISA being determined by using 22 fecal samples from healthy dogs, 84 fecal samples from diarrhoeic dogs were examined for CCV infection. The results showed that 16 of 84 samples were CCV positive. Meanwhile, Dot ELISA demonstrated that 13 out of 84 were CCV positive. These 13 samples were included in those 16 samples which were positive in sandwich ELISA. 38 out of 84 diarrhoeic fecal samples were tested for both CCV and canine parvovirus (CPV) infection. Among them, 16 samples were also CCV positive, 6 were CPV positive, 4 of 6 CPV positive samples were also CCV positive. These results suggested that CCV infection might be more important than CPV infection in the kennels in Nanjing city.

关 键 词：犬冠状病毒 夹心ELISA DOT-ELISA 

分 类 号：S852.655[农业科学—基础兽医学] S854.43[农业科学—兽医学]