猪带绦虫六钩蚴抗原基因的克隆与表达  

Cloning and Expression of the Genome from Taenia solium Oncospheres

作　　者：翟春生[1] 唐雨德[1] 顾志香[1] 

机构地区：[1]南京军区军事医学研究所

出　　处：《中国兽医学报》1997年第5期470-472,共3页Chinese Journal of Veterinary Science

摘　　要：以λｇｔ１１为载体构建了猪带绦虫六钩蚴ｃＤＮＡ文库，从中筛选出５株表达六钩蚴抗原成分的重组噬菌体。将其中的六钩蚴ｃＤＮＡ与表达质粒ｐＧＥＸ－４Ｔ２连接，在ＴＧ１大肠杆菌中高效表达，获得了２株表达ＧＳＴ－六钩蚴抗原融合蛋白（Ｍｒ分别为５×１０４和６×１０４）的工程菌。在ＬＢ营养液中添加１％乳糖和０．２％葡萄糖，工程菌的表达效果优于用ＩＰＴＧ诱导的效果。融合蛋白约占工程菌总蛋白的２３％。A cDNA library was constructed in the vector λgt11 from Taenia solium oncospheres mRNA, and screened for bacteriophage colonies producing products immunologically related to Taenia solium oncosphere proteins by incubating the NC filters with a rabbit anti Taenia solium oncosphere antibody and then with HRP conjugated goat anti rabbit IgG. 5 positive clones were identified and subcloned in the expression plasmid pGEX 4T 2 for producing fusion protein of GST oncosphere antigen in E. coli TG 1. Five recombinant fusion proteins were differentiated to two compositions, their molecular weights were about 5×10 4 and 6×10 4, respectively. When 1% lactose and 0.2% glucose were added to LB medium, the expression of the recombinant bacteria was more efficient than that IPTG was added. The fusion proteins were about 23% of the total proteins of the recombinant bacteria.

关 键 词：猪带绦虫 CDNA文库 基因克隆 融合表达 

分 类 号：S858.285.9[农业科学—临床兽医学] Q78[农业科学—兽医学]