氮离子注入生物絮凝剂产生菌的诱变效应研究  

Mutagenic effects of bioflocculant-producing strains by N^+ ion implantation

作　　者：李培睿[1] 闫世梁[1] 张东辉[1] 李宗义[2] 秦广雍[1] 霍裕平[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,郑州450052 [2]河南师范大学生命科学学院,新乡453002

出　　处：《核技术》2008年第3期193-196,共4页Nuclear Techniques

基　　金：国家重点基础研究发展规划973项目(2004CB719604)资助

摘　　要：采用30keV氮离子注入对生物絮凝剂产生菌进行诱变选育,研究低能氮离子对微生物的诱变效应。结果表明:FJ-7菌株的存活率曲线为典型的"马鞍型"剂量-效应曲线。经5×1015ions·cm-2N+注入诱变处理,最终获得一株絮凝活性高、遗传稳定性良好的突变株NIM-192。其发酵产絮凝剂曲线表明,突变株NIM-192的菌体生长速度稍慢于原始菌株,但其絮凝活性一直高于原始菌株,絮凝率比原始菌株提高了34.26%。这很可能是离子注入菌体后所产生的生物学效应使得菌体合成更多的絮凝剂,导致絮凝活性的增强。The bacteria of bioflocculant-producing Brevundimonas sp.FJ-7 was implanted by 30 keV N^＋＊ ions to a doses of 5×10^14 -1×10^16 ions·cm^2. Mutagenic effects of the microbe were studied. The results indicated that the survival rates exhibited a saddle-like curve and the mutagenic effects of N^＋ ion implantation were unique. A high genetic stability and flocculating activity mutant strain, NIM-192 was screened out, with a dose of 5×10^15 ions·cm^2. Fermentation curve of bioflocculant-producing showed the growing speed of NIM-192 was slower, but the flocculating activ- ity was higher than the original one, and the flocculating rate of NIM-192 increased by 34.26%. This indicated that biological effects produced by N^＋ ion implantation promoted the synthesis of the bioflocculant and increased the flocculating activity.

关 键 词：N^+注入 生物絮凝剂 诱变选育 诱变效应 

分 类 号：Q937[生物学—微生物学]