白菜胞质雄性不育系叶绿体DNA的SSR分析  

SSR Analysis of Chloroplast DNA of Pol Cytoplasmic Male Sterile Lines in Chinese Cabbage

作　　者：邱正明[1] 邓晓辉[2] 聂启军[2] 朱凤娟[2] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,武汉430072 [2]湖北省农业科学院蔬菜科技中心,武汉430064

出　　处：《湖北农业科学》2008年第2期134-137,共4页Hubei Agricultural Sciences

基　　金：国家"863"计划项目(2003AA207120)

摘　　要：从烟草的36对叶绿体SSR引物中筛选出10对引物对白菜不育系和保持系的叶绿体基因组进行了SSR分析,发现两系间扩增片段的多态性较小,获得了1条只在不育系稳定出现的微卫星序列。此结果显示,不育系和保持系的叶绿体基因组间确实存在差异,此差异也许与细胞质雄性不育相关。Chloroplast simple sequence repeat （cpSSR） was developed and used to analyze chloroplast genome difference of a Pol cytoplasmic male sterile （CMS） line and its maintainer line of Chinese cabbage. Thirty-six pairs of cpSSR primer from tobacoo （Nicotiana. tobocum L.） were tested in Chinese cabbage, ten of which could amplify intensive PCR products by polyacrylamide gel electrophoresis. Genome of a CMS line and its maintainer line was then analyzed. The result showed that there was little polymorphism and one special fragment was present only in CMS line. These results revealed the difference of the chloroplast genome between CMS line and its maintainer line, which likely related to CMS.

关 键 词：白菜 胞质雄性不育 叶绿体 微卫星技术 

分 类 号：S634.3[农业科学—蔬菜学] Q343.34[农业科学—园艺学]