绒山羊胚胎期和兴盛期皮肤毛囊差异表达基因研究  

Study on Differently Expressed Genes in Skin Follicle of Cashmere Goat at the Embryonic Stage and Anagen Stage

作　　者：李玉荣[1] 尹俊[2] 张燕军[1] 刘羿羿[1] 李金泉[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院 [2]内蒙古农业大学生物工程学院,呼和浩特010018

出　　处：《湖北农业科学》2008年第2期146-148,共3页Hubei Agricultural Sciences

基　　金：内蒙古自然科学基金重大项目(200408020401)

摘　　要：应用抑制性消减杂交技术成功构建了内蒙古绒山羊胚胎期和兴盛期皮肤毛囊的消减cDNA文库。从两个文库中分别随机挑取50个克隆测序分析,测序结果经Genbank同源性比对,表明在胚胎期消减cDNA文库中得到15个特异表达基因片段,包含1个未知新序列;在兴盛期消减cDNA文库中得到19个特异表达基因片段,包含2个未知新序列。The skin follicle subtractive cDNA library of the Inner Mongolian cashmere goat was constructed by suppression subtractive hybridization. 50 clones which were derived from the embryonic stage and anagen eDNA library respectively were sequenced and blasted with known gene in Genbank. 15 cDNA fragments were obtained from the embryonic stage eDNA library, 14 fragments corresponded to known gene and one was unknown sequence. 19 eDNA fragments were obtained from anagen eDNA library,17 cDNA fragments corresponded to known gene and two was unknown sequence .

关 键 词：抑制性消减杂交 绒山羊 胚胎期 兴盛期 消减cDNA文库 

分 类 号：Q503[生物学—生物化学] S827.91[农业科学—畜牧学]