金龟子绿僵菌代谢产物成分预试验及其生物碱的获得  

Preliminary Test of Metabolic Product and Alkaloids Obtaining in Metarhiziumanisopliae

作　　者：孔平[1] 李倩[1] 李永娟[1] 唐啸华[1] 杨鸣琦[1] 

机构地区：[1]西北农林科技大学动物医学学院,陕西杨凌712100

出　　处：《湖北农业科学》2008年第2期226-228,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金资助项目(30270979);陕西省自然科学基金资助项目(2003C119)

摘　　要：参照植物化学成分系统预试的方法对金龟子绿僵菌发酵代谢产物进行了初步分析;在pH值≥10的条件下,采用萃取技术、TLC和紫外分光光度法对代谢产物中的总生物碱进行了提取、分离和鉴定。结果表明,代谢产物中苦马豆素的特征显色反应明显;TLC、紫外分光光度法检测发现升华后的产物中以苦马豆素为主要成分,为苦马豆素获取途径的优化提供了新思路。Preliminary analysis of products of the fermental Metarhiziumanisopliae was done in accordance with chemical systematic test of plant. On the condition pH〉10, extraction,TLC and ultraviolet spectrophotometry were used to get and appraise the total alkaloids from the metabolic product. The result showed that characteristic colour response of swainsonine was conspicuous.SW was the essential component in the sublimate.

关 键 词：金龟子绿僵菌 发酵 成分预试 苦马豆素 TLC 紫外分光光度法 

分 类 号：S763.38[农业科学—森林保护学] TQ464.4[农业科学—林学]