大鼠脊髓损伤后运动功能的恢复及前角运动神经元功能的改变  

Recovery of Motor Function and Changes of Anterior Horn Cells after Spinal Cord Injury in Rats

作　　者：欧喜超[1] 杨朝阳[1] 刘玉军[1] 鲁强[1] 张皑峰[2] 李晓光[1] 

机构地区：[1]首都医科大学北京神经科学研究所,北京市神经再生修复研究重点实验室,教育部神经变性病学重点实验室,北京市100069 [2]首都医科大学附属友谊医院口腔科,北京市100050

出　　处：《中国康复理论与实践》2008年第3期231-233,F0003,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市自然科学基金项目(7041002);北京市教育委员会科技发展计划项目(KZ200310025008)

摘　　要：目的观察大鼠不同方式脊髓损伤后损伤下段脊髓前角运动神经元内降钙素基因相关肽（CGRP）与乙酰胆碱酯酶（AChE）的变化,并对损伤后后肢运动功能的恢复情况进行比较。方法60只成年Wistar大鼠随机分为假手术组、切割型脊髓损伤组和撞击型脊髓损伤组,采用联合均分（ACOS）神经行为学评定观察损伤后大鼠运动功能的恢复,术后1、2、4、10周,用Karnovsky-Roots直接法染色检测L2～L4段脊髓AChE、免疫组化方法检测CGRP的水平。结果术后1～10周内,撞击组大鼠ACOS得分明显高于切割组,两损伤组大鼠术后前角运动神经元CGRP和AChE的含量均下降,但撞击组10周时有了明显恢复,而切割组一直处于较低水平,且CGRP的改变早于AChE。结论在大鼠不同脊髓损伤后,运动功能变化与前角运动神经元的功能状态具有一定联系;CGRP和AChE可能在对脊髓损伤后运动功能的恢复中起一定作用。Objective To explore the restoration of motor function and the expression of calcitonin gene-related peptide（CGRP）and acetylcholine esterase（AChE）in the anterior horn motoneurons after different types of spinal cord injury.Methods 60 adult female Wistar rats were randomly assigned to 3 groups：sham group,completely transection group and contusion group.Average combined scores（ACOS）were applied to assess the motor function at various time after the surgery.The content of AChE in the anterior horn of L2-L4 was detected with Karnovsky-Roots staining and the expression of CGRP was then determined with immunohistochemistry.Results The scores of ACOS were much higher in the contusion group than in the transection group at each time point examined.The content of both AChE and CGRP significantly decreased after either type of spinal cord injury.However,their activity gradually recovered to the normal level in the contusion group,but not in the transection group.Moreover,the changes of CGRP occurred earlier than those of AChE.Conclusion There is strong relationship between the motor function recovery and the functional state of anterior horn cells.CGRP or AChE may play an important role in the functional recovery of locomotion after spinal cord injury in rats.

关 键 词：脊髓损伤 评定 运动功能 降钙素基因相关肽(CGRP) 乙酰胆碱酯酶(AChE) 大鼠 

分 类 号：R651.2[医药卫生—外科学]