氟对大鼠胆碱酯酶活性和学习记忆功能的影响  

Effects of fluoride on learning and memory and cholinesterase activity in rat brains

作　　者：高勤[1] 刘艳洁[1] 吴昌学 龙义国[1] 官志忠[1] 

机构地区：[1]贵阳医学院病理学教研室,550004 [2]分子生物学重点实验室

出　　处：《中国地方病学杂志》2008年第2期128-130,共3页Chinese Jouranl of Endemiology

基　　金：科技部973项目（2006CB708513）;国家自然科学基金（30760224）;贵州省科技厅项目[黔科合外G字（2006400107）、黔省专合字（2006）52、黔科合重大专项（2006）6015]

摘　　要：目的研究氟对慢性氟中毒大鼠学习、记忆功能的影响以及其可能的机制，探讨氟对脑组织胆碱酯酶活性的影响和氟中毒大鼠智力损伤程度的关系。方法SD大鼠按性别和体质量随机分为3组，对照组：自由饮用自来水，含氟量低于1mg／L；低剂量染氟组：饮水含氟量为5mg／L；高剂量染氟组：饮水含氟量为50mg／L。实验3个月时检测大鼠行为学变化和脑组织胆碱酯酶活性。结果高剂量染氟组大鼠的逃避潜伏期时间[（17．55±1．51）s]长于对照组[（12．07±0．97）s]，两组比较差异有统计学意义（P〈0．05）；高剂量染氟组探索实验目标次数[（2．88±0．35）次]与对照组[（4．00±0．50）次]比较，有降低趋势，但差异无统计学意义（P〉0．05）；高剂量染氟组的乙酰胆碱酯酶和丁酰胆碱酯酶活性[（1．41±0．19）、（0．49±O．07）kU／g]均低于对照组[（1．88±0．13）、（1．04±0．16）kU／g）]，差异均有统计学意义（P〈0．05）。结论过量氟在体内蓄积可使大鼠智力降低，脑内的胆碱酯酶活性降低可能是其机制之一。Objective To study the role of fluorosis on learning and memory in rat and its possible mechanisms; to explore the correlation between cholinesterase activity and the level of intelligence. Methods SD rats divides into 3 group of stochastically according to the sex and the body weight, control group treated with drinking water containing fluoride less than 1 rag/L, lower fluoride exposed group with drinking water containing fluoride 5 rag/L, and higher fluoride exposed group with drinking water containing fluoride 50 mg/L. The ability of learning and memory and cholinesterase activity were detected in the third month after experiment. Resets Escape latency in higher fluoride exposed group[ （17.55± 1.51 ）s]was significantly higher than that of controls group [ （12.07± 0.97）s], the difference being statistically significant （P 〈 0.05 ）. The numbers of crossing platforms in higher fluoride exposed group[ （2.88 ± 0.35）times] were lower than those of controls group[ （4.00 ± 0.50）times], but the difference was not statistically significant（P 〉 0.05 ）. The activities of acetylcholinesterase and butylcholinesterase in higher fluoride exposed group [ （1.41 ± 0.19）, （0.49 ± 0.07 ）kU/g ] were significantly lower than those of controls [ （1.88 ± 0.13）, （1.04± 0.16）kU/g], the difference had statistical significances （P 〈 0.05）. Conclusions The results indicate that excessive fluoride can decrease the level of intelligence of rats, the declined activities of cholinesterase may be the possible pathogenetic mechanism.

关 键 词：氟化物中毒 学习 记忆 乙酰胆碱酯酶 丁酰胆碱酯酶 

分 类 号：R686[医药卫生—骨科学]