5种黄酮类化合物对凋亡心肌细胞Bcl-2家族蛋白表达的影响  

Effects of five flavonoids on expression of Bcl-2 family proteins in apoptosis of myocardiocytes

作　　者：张锦[1] 赵翠翠[1] 陆景坤[1] 王丽伟[1] 高梓真[1] 韩维娜[1] 刘凤芝[1] 

机构地区：[1]哈尔滨医科大学药物化学与天然药物化学教研室,150081

出　　处：《中国地方病学杂志》2008年第2期149-151,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金（30371684）;黑龙江省自然科学基金（D0301）;哈尔滨医科大学医学基础学科创新群体基金（2007）

摘　　要：目的观察5种黄酮类化合物芦丁（RU）、二氢杨梅素（DMY）、陈皮素（HP）、大豆黄素（DA）、红花黄色素A（HYSA）对H202诱导的心肌细胞凋亡的作用，探讨其与克山病的关系及可能的作用机制。方法培养新生Wistar仔鼠心肌细胞，分为对照组、模型组、药物孵育组，用荧光染色法观察心肌细胞凋亡情况，流式细胞仪检测细胞凋亡率，Western blot法观察凋亡相关基因Bcl-2家族蛋白表达。结果与模型组[（24．33±6．51）％]比较，RU[（13．95±3．80）％]、DA[（11．82±3．50）％]、HYSA[（12．33±3．78）％]能有效降低心肌细胞凋亡率（P〈0．05）。5种黄酮类化合物能不同程度地上调Bcl-2蛋白表达，下调Bax蛋白表达，使Bcl-2／Bax比值增加[RU（0．989±0．094）、DMY（0．931±0．280）、HP（0．980±0．095）、DA（1．049±0．092）、HYSA（1．031±0．039）]，与模型组（0．490±0．046）比较，差异均有统计学意义（P〈0．05）；而Bcl-xl表达无明显变化（P〉0．05）。结论RU、DMY、HP、DA、HYSA可以通过Bcl-2、Bax途径调节细胞凋亡过程，发挥抗氧化作用，抑制细胞凋亡，为克山病防治提供了一种思路。Objective To observe the effects of five flavonoids include rutin（RU）, dihydromyricetin（DMY）, hesperetin（HP）, daidzein （DA） and hydroxysaffor yellow A（HYSA） on myocardiocyte apoptosis induced by H2O2 and to explore their relationships with Keshan disease and the possible mechanism. Methods Primary cultured cardiocytes of neonatal rats were randomly divided into control group, model group, and flavonoids preincubation group. The cardiocyte apoptosis was examined by fluorescent staining, the rates of apoptosis were detected by flow cytometry, the expression of Bcl-2 family proteins associated with apoptosis were observed by Western blot. Results Compared with model group[（24.33±6.51）%], RU[（13.95 ± 3.80）%], DA[（11.82 ± 3.50）%], HYSA[ （12.33± 3.78）% ] could decreased the rate of apoptosis （P 〈 0.05）. The five flavonoids could up-regulate Bcl-2 expression, down-regulate Bax expression, and increase the Bcl-2/Bax ratio[RU（0.989 ± 0.094）, DMY （0.931 ±0.280）, HP（0.980± 0.095）, DA（1.049 ± 0.092）, HYSA（1.031±0.039）, vs model（0.490 ± 0.046）, the difference had statistical significances（P 〈 0.05 ）], but the Bcl-xl did not significantly changed（P 〉 0.05 ）. Conclusions RU, DMY, HP, DA and HYSA have antiapoptotic effects on cardiomyocyte via regulating Bcl-2 and Bax, which gives us a hint in prevention and treatment of Keshan disease.

关 键 词：克山病 黄酮类 肌细胞 心脏 细胞凋亡 基因 BCL-2 

分 类 号：R686[医药卫生—骨科学]