南瓜蛋白与STI571体外联合抗K562细胞的作用  

The Effect of Cucurmosin and STI571 on K562 Cell Growth in vitro

作　　者：孙建成[1] 谢捷明[1] 张昆仲[1] 黄自强[1] 陈明晃[2] 

机构地区：[1]福建医科大学药学院,福州350004 [2]中国科学院福建物质结构研究所,结构化学国家重点实验室,福州350002

出　　处：《福建医科大学学报》2008年第1期30-32,41,共4页Journal of Fujian Medical University

基　　金：国家自然科学基金资助项目(30772587);福建省自然科学基金资助项目(C0510012);福建医科大学科学研究发展基金资助项目(FJGXY04019)

摘　　要：目的探讨南瓜蛋白与STI571对K562细胞增殖的抑制作用及其机制。方法用MTT检测南瓜蛋白与STI571单用或联合作用对K562细胞增殖的抑制作用;流式细胞仪检测药物对细胞的凋亡率;Westernblot检测药物对p210Bcr-Abl蛋白的影响。结果南瓜蛋白与STI571联合作用协同抑制K562细胞增殖(q>1.15);协同诱导K562细胞凋亡;协同抑制p210Bcr-Abl蛋白表达。结论南瓜蛋白与STI571联合作用体外协同抗K562细胞的增殖。Objective To investigate the effect and mechanism of cucurmosin and STI571 on K562 cell growth. Methods MTT was used to evaluate the growth inhibitory effect of cucurmosin and STI571. FCM was used to detect the apoptotic rate induced by cucurmosin and STI571. Western blot was used to investigate the effect of p210^Bcr-Abl induced by cucurmosin and STI571. Resolts Cucurmosin and STI571 exerted a synergistic inhibitory effect on K562 cell growth（q〉1. 15）. FCM showed that cucurmosin and STI571 also exerted a synergistic apoptotic effect on K562 cells（q〉1. 15）. Western blot indicated cucurmosin and STI571 synergistically inhibited the expression of p210^Bcr-Abl. Oonclusion Cucurmosin enhances STI571 in inhibition of K562 cell growth.

关 键 词：南瓜 K562细胞 植物蛋白类 受体蛋白质酪氨酸激酶类 细胞增殖 

分 类 号：R285[医药卫生—中药学]