异基因骨髓源间充质干细胞对BXSB小鼠T、B细胞的影响  

The immunoregulatory effects of allogeneic BMSCs on T and B cells from BXSB mice

作　　者：赵慧丰[1] 高燕[2] 杜涌瑞[2] 韩钦[3] 廖联名[3] 赵春华[3] 邓鸿业[4] 姚智[1] 邓为民[1] 

机构地区：[1]天津医科大学基础医学院免疫学教研室天津市细胞与分子免疫学重点实验室,天津300070 [2]天津市中心妇产科医院,天津300082 [3]中国医学科学院协和医学院组织工程研究中心,北京100005 [4]北京大学医学部免疫学系,北京100083

出　　处：《中国免疫学杂志》2008年第3期195-199,共5页Chinese Journal of Immunology

基　　金：863计划(2002AA205061);国家自然科学基金(30670801);天津市科委基金(06YFJMJC08300)资助

摘　　要：目的:探讨异基因骨髓源间充质干细胞(Bone marrow derived mesenchymal stem cells,BMSCs)对BXSB小鼠T、B细胞增殖、活化及功能成熟等方面的影响。方法:采用3H-TdR掺入法、FACS法、ELISA等方法检测BMSCs对BXSB小鼠T、B细胞的影响。结果:BALB/c小鼠的BMSCs在不影响BXSB小鼠T细胞诱导活化的基础上抑制其诱导增殖;可降低由ConA诱导的CD4+IL-4+细胞的数量,而提高由ConA诱导的CD4+IFN-γ+细胞数量。对于B细胞,BALB/c小鼠的BMSCs可以抑制其增殖、活化及IgG的分泌。另外,BALB/c小鼠的BMSCs可以抑制BXSB小鼠B细胞上CD40L的异位表达。结论:异基因BMSCs对自身免疫病小鼠的T、B细胞有一定的调节作用。Objective：To investigate the immunoregulatory effects of allogeneic BMSCs on T and B cells from BXSB mice with SLE. Methods： In this work,^3H-TdR incorporation assay, ELISA, FACS were performed to evaluate the effects of BMSCs on immunocompetent cells of BXSB mice. Results： BMSCs from BALB/c had the inhibitory effects on BXSB mice T lymphocytes proliferation without affecting their activation;Significant inhibition on ConA-induced CD4^＋ IL-4^＋ producing cells and enhancing effects on CD4^＋ IFN-γ^＋ producing cells;As to B lymphocytes of BXSB mice, BMSCs had inhibitory effects on their proliferation, activation and IgG secretion; In addition, BALB/c BMSCs had inhibitory effects on CD40 hgand （CD40L） ectopic hyper-expression on B cells of BXSB mice. Conclusion： Allogeneic BMSCs had some immunoregulatory effects on T and B cells of BXSB mice with SLE.

关 键 词：异基因 骨髓源间充质干细胞 免疫调节 免疫活性细胞 SLE 

分 类 号：R392.3[医药卫生—免疫学]