钙拮抗剂对大鼠心肌缺血再灌注早期生长反应基因-1表达的影响  

Effects of Calcium Atagonist on Early Growth Response Gene-1 Expression in Rats after Myocardial Ischemia-Reperfusion

作　　者：李海青[1] 石刚刚[1] 高分飞[1] 贾强用[1] 

机构地区：[1]汕头大学医学院药理学教研室,广东汕头515041

出　　处：《汕头大学医学院学报》2008年第1期7-10,共4页Journal of Shantou University Medical College

基　　金：国家自然科学基金资助项目(30672465);广东省自然科学基金重点项目(06118928)

摘　　要：目的:研究钙拮抗剂(维拉帕米、地尔硫和硝苯地平)对大鼠心肌缺血再灌注(I/R)早期生长反应基因-1(Egr-1)表达的影响。方法:大鼠随机分为假手术组、I/R组、二甲基亚砜组、维拉帕米组、地尔硫组、硝苯地平组。Western blot法测定Egr-1蛋白表达水平的变化;RT-PCR法测定Egr-1 mRNA表达水平的变化。结果:与假手术组比,I/R组Egr-1蛋白及Egr-1 mRNA表达显著增高(P<0.05);与I/R组比,维拉帕米组、地尔硫组和硝苯地平组Egr-1蛋白及Egr-1 mRNA表达明显减少(P<0.05)。结论:维拉帕米、地尔硫和硝苯地平均可抑制缺血再灌注心肌Egr-1蛋白及Egr-1 mRNA的过度表达。Objective： To study the effects of calcium antagonist（verapamil, diltiazem and nifedipine）on early growth response gene-1（Egr-1）expression in rats after myocardial ischemia-reperfusion（I/R）. Methods： Rats were randomly assigned into Sham, I/R, DMSO, vempamil, diltiazem, nifedipine groups. The expression levels of Egr-1 protein and mRNA were examined by Western blot and RT-PCR, respectively. Results： Compared with sham group, Egr-1 protein and mRNA of I/R group overexpressed（ P 〈 0.05）. The expression levels of Egr-1 of vempamil, diltiazem, nifedipine groups significantly decreased, compared with I/R group（ P 〈 0.05）. Conclusion： Verapamil, diltiazem and nifedipine ean inhibit the overexpression of Egr-1 protein and mRNA in rots caused by I/R.

关 键 词：钙拮抗剂 缺血再灌注 早期生长反应基因-1 

分 类 号：R966[医药卫生—药理学]