Cloning and Sequence Analysis of a Full-length cDNA Encoding Light-harvesting Complexes of Photosystem II in Phyllostachys edulis  

作　　者：LIU Yingli GAO Zhimin PENG Zhenhua YUE Yongde 

机构地区：[1]International Center for Bamboo and Rattan Key Laboratory on Bamboo and Rattan Science and Technology of State Forestry Administration, Beijing 100102, P.R. China [2]Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 P.R. China

出　　处：《Chinese Forestry Science and Technology》2007年第4期47-52,共6页中国林业科技（英文版）

基　　金：This project is supported by "948" Introduction Project (2004-4-60, 2005-4-38)

摘　　要：The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （Phyllostachys edulis） cDNA through RT-PCR and RACE methods, named as cabPhEIO （cab gene 10 from Ph. edulis）. The length of cab- PhEIO （GenBank accession number： EU118754） is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhElO had a chlorophll a/b binding domain （83rd -247th position）, two protein kinase C-phosphorylation sites, three Nmyristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhElO showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhElO gene belongs to lhcb5 gene family.The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b (cab) gene was cloned from the first strand of Moso (Phyllostachys edulis) cDNA through RT-PCR and RACE methods, named as cab- PhE10 (cab gene 10 from Ph. edulis). The length of cab- PhE10 (GenBank accession number: EU118754) is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhE10 had a chlorophll a/b binding domain (83rd -247th position), two protein kinase C-phosphorylation sites, three N- myristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhE10 showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhE10 gene belongs to lhcb5 gene family.

关 键 词：Moso （Phyllostachys edulis （Caar.） H.de Lehaie） light-harvesting complexes （LHC） photosystems II（PS II） RT-PCR RACE sequence analysis 

分 类 号：S81[农业科学—畜牧学]