复智散色谱指纹图谱的初步研究  

Study on Fingerprinting of Fu Zhi San

作　　者：赵敬堃[1] 王德生[1] 王建秀[1] 段淑荣[1] 李文兰[2] 白晶[2] 

机构地区：[1]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001 [2]哈尔滨商业大学药物研究所,黑龙江哈尔滨150086

出　　处：《中国实验方剂学杂志》2007年第11期4-7,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金资助(30670726)

摘　　要：目的:建立复智散反相高效液相色谱(RP-HPLC)指纹图谱的分析方法。方法:采用RP-HPLC法,线性梯度洗脱,流动相为乙腈-0.1%磷酸水溶液,体积流量1.0 mL.min-1,检测波长335 nm,柱温30℃。实验测定多批样品并记录指纹图谱,不同样品之间的相似度采用国家药典委员会中药色谱指纹图谱相似度评价系统研究版(2004A)计算。结果:精密度、重复性、稳定性试验中相对共有峰面积和相对保留时间的RSD均小于2%。在选定的色谱条件下确定了19个峰为复智散的指纹特征峰,自制样品平均相似度为0.996。结论:该法精密度高,重复性好,简便实用,可为复智散的指纹图谱评价提供依据。Objective： To establish the chromatographic fingerprints of Fu Zhi San by RP-HPLC. Methods： By RP-HPLC, acetonitrile and water including 0.1% Phosphoric acid were mobile phase with gradient elution,flow rate was 1.0 mL·min^-1, the detection wavelength was 335 nm, and the temperature was 30℃. The fingerprints of different samples were compared with similarity evaluation software published by committee codex. Results：The RSD of relative peak areas and relative retention time of common peak in the test of precision, repeatability, and stability has not reached 2 %, respectively. 19 peaks were identified as the characteristic fingerprints. Average similarity was 0.996. Conclusion： This method is precise, practicable, well-repeated and simple. It can be used as the method to judge the quality of Fu Zhi San .

关 键 词：复智散 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]