胞外调节蛋白激酶在糖尿病肾病中激活机制及健脾益肾活血中药的干预作用  

Experimental Study on Mechanism of ERK Activity and intervention role of Chinese Herb Recipe in Early Diabetic Nephropathy Rats

作　　者：关欣[1] 郑红光[1] 徐爱华[2] 

机构地区：[1]解放军沈阳军区总医院,辽宁沈阳110016 [2]辽宁医学院,辽宁锦州121000

出　　处：《云南中医学院学报》2007年第6期24-27,共4页Journal of Yunnan University of Traditional Chinese Medicine

摘　　要：目的:观察健脾益肾活血中药对糖尿病肾病(DN)大鼠肾皮质中磷酸化胞外调节蛋白激酶(P-ERK)、转化生长因子(TGF-β1)基因、血小板衍化生长因子(PDGF-B)基因的表达及肾脏病理变化的影响。探讨ERK被激活的分子机制及中药复方对DN肾脏的保护作用机制。方法:雄性Wistar大鼠33只,腹腔注射链脲佐菌素建立早期DN模型。将大鼠随机分为正常组、模型组、中药治疗组。观察6周。检测血糖、肾肥大指数,用Western Blot检测肾皮质P-ERK;用RT-PCR技术检测TGF-β1mRNA、PDGF-BmRNA的表达。结果:治疗后6周模型组和治疗组血糖值、肾肥大指数和肾小球系膜细胞基质(ECM)相对面积均明显高于正常组(P值均小于0.01)。治疗组血糖值、肾肥大指数、(ECM)相对面积低于模型组,(P值均小于0.01)。治疗组肾皮质P-ERK、TGF-β1mRNA、PDGF-BmRNA的表达均明显低于模型组,肾组织病变明显减轻。结论①ERK参与了DN的发生与发展。②中药复方抑制DN大鼠肾皮质中ERK的活性,并下调TGF-β1mRNA、PDGF-BmRNA的过度表达,减轻肾组织病理改变;③中药复方对DN肾脏的保护作用,可能与抑制ERK活性有关。④ERK活化与DN高血糖、TGF-β1RNA及PDGF-BmRNA的过度表达有关。Objective： To observce the effect of Chinese Herb recipe on the expression of ERK1/2, TGF - β1 gene, PDGF - B gene in early diabetic nephropathy rats, try to explore the possible mechanism of ERK1/ 2 activity and the protection role of Chinese Herb recipe on diabetic nephropathy. Methods： The diabetic nephropathy model of the rats were established by intra-abdominal injection of STZ. The experimental rats were randomly divided into normal contrast group, pattern group and treated group. Then the rats were treated with Chinese Herb recipe for six weeks. Blood glucose and kidney hypertrophy index were measured. The level of phospho-ERK1/2 was examined by western blot. TGF - β1 gene and PDGF - B gene were measured by RT-PCR. Results： Blood glucose, Kidney hypertrophy index and Extracelluar matrix of modern group and treated group were significantly higher than blood glucose, kidney hypertrophy index and extracelluar matrix of normal group after 6 weeks （P 〈 0. 01 ）. Treated group were lower than those of modern group （P 〈 0. 01 ）. The expression of pERK1/2, gene of TGF - β1 and PDGF - B of renal cortex in treated group were significantly lower than that of in modern group. Chinese Herb recipe can relieve the pathological change of renal tissue. Conclusions ： （1）pERK1/2 was participated in the occurring and developing of diabetic nephropathy. （2）Chinese Herb recipe can down-regulate hyper-expression of gene of TGF - β1 and PDGF - B in renal cortex. （3）It may have relation with inhibiting the activity of pERK1/2 that Chinese Herb recipe can treat early diabetic nephropathy. （4）The activity of pERK1/2 may have relation with the hyperexpression of gene of TGF - β1 and PDGF - B of renal cortex.

关 键 词：健脾益肾活血中药 DN大鼠 P—ERKI/2 肾皮质TGF—β1mRNA PDGF—B 

分 类 号：R255.4[医药卫生—中医内科学]