糖尿病大鼠视网膜中缺氧诱导因子-1α和血管内皮生长因子表达的研究  

Expression of hypoxia-inducible factor-1α and vascular endothelial growth factor in the retina of diabetic rats

作　　者：徐国兴[1] 许建斌[1] 胡建章[1] 

机构地区：[1]福建医科大学附属第一医院福建省眼科研究所,中国福建省福州市350005

出　　处：《国际眼科杂志》2008年第3期487-490,共4页International Eye Science

基　　金：中国卫生部攻关课题基金资助项目(No.WKJ2005-2-013);中国福建省自然科学科研课题基金资助项目(No.C0510015);中国福建省教育厅科研基金资助项目(No.JB05244)~~

摘　　要：目的:检测缺氧诱导因子-1α(HIF-1α)与血管内皮生长因子(VEGF)在糖尿病大鼠视网膜中的表达,探讨其在糖尿病视网膜病变(DR)发生发展中的作用。方法:SD大鼠120只随机分为对照组与DR组,DR组采用链脲佐菌素(STZ)一次性建立糖尿病模型。应用免疫组化SP法和RT-PCR技术分别于1,3,6mo检测视网膜中HIF-1α与VEGF蛋白及其mRNA的表达变化。结果:HIF-1α与VEGF蛋白及其mRNA在对照组视网膜中不表达,在DR组中呈进行性表达增强(P<0.05);DR组中HIF-1α与VEGF的表达呈正相关(r=0.605,P<0.05)。结论:DR视网膜中HIF-1α与VEGF表达增强,与DR的发生发展密切相关。AIM： To detect the expression of hypoxia-inducible factor- 1α （HIF-1α） and vascu1αr endothelial growth factor （VEGF） in the retina of diabetic rats, and to investigate their roles in the pathogenesis of diabetic retinopathy （DR）. METHODS： One hundred and twenty SD rats were randomly divided into two groups： DR group and control group. Diabetes was induced by streptozotocin （STZ） intraperitoneal injection in DR group. The techniques of immunohistochemistry and reverse transcription poly- merase chain reaction （RT-PCR） were used to examine the expression of HIF-1α, VEGF protein and mRNA in retina respectively at 1,3,6 months after STZ injection. RESULTS. The results of immunohistochemical technique and RT-PCR showed that the expression of HIF-1α, VEGF protein and mRNA in retina were not found in control group, but were found gradually increased in DR group （ P 〈 0.05）. There was a positive corre1αtion between the expression of HIF-1α and that of VEGF（r=0.605,P〈0.05）. CONCLUSION, HIF-1α may regu1αte the expression of VEGF, and both are involved in the pathogenesis of DR.

关 键 词：糖尿病视网膜病变 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R587.2[医药卫生—内分泌] R735.34[医药卫生—内科学]