乙肝患者肝组织中HBVcccDNA与血清中HBVDNA、HBeAg的关系  

Liver Tissue CCCDNA and Serum HBV DNA,HBeAg in Patients with Hepatitis B Correlation Between

作　　者：张永乐[1] 徐岱[1] 朱国献[1] 吴晓星[1] 武静[1] 邵芯仪[1] 陈虹[1] 张勤[1] 

机构地区：[1]浙江中医药大学附属杭州市第六人民医院,310014

出　　处：《医学研究杂志》2008年第3期60-61,共2页Journal of Medical Research

基　　金：杭州市科技局科研项目(2004433Q03);杭州市卫生局重点传染专科课题

摘　　要：目的探讨乙肝患者肝组织中HBVcccDNA与血清中HBVDNA、HBeAg的关系。方法采用实时荧光定量聚合酶链反应检测乙肝患者肝组织中HBVcccDNA及血清HBVDNA,同时采用ELISA检测其血清中免疫指标HBeAg。结果30例HB-sAg、HBeAg、抗HBc阳性的乙肝患者血清中HBVDNA阳性28例阳性率93.33%,肝组织中HBVcccDNA阳性19例阳性率63.33%,两者比较差异有显著性P<0.05。30例HBsAg、抗HBe、抗HBc阳性的乙肝患者血清中HBVDNA阳性8例阳性率26.67%,肝组织中HBVcccDNA阳性5例阳性率16.67%,两者比较差异无显著性P>0.05。10例脂肪肝患者血清HBVDNA阴性,肝组织中未检出HBVcccDNA。结论(1)血清HBVDNA或HBeAg阳性并不能完全确定肝内一定存在乙肝病毒正在复制。(2)HBeAg阴性抗HBe阳性不能完全确定肝内一定没有乙肝病毒复制。(3)要确定肝内病毒是否存在复制必须通过肝组织HB-VcccDNA检测来证实。Objective To explore the correlation between liver tissue cccDNA and serum HBV DNA, HBeAg in patients with hepatitis. Methods Hepatitis B patients liver tissue cccDNA and serum HBV DNA were defected by Real - time fluorescence quantitative polymerase chain reaction,while immune parameters of HBeAg in Serum was defeefed by ELISA. Results Of 30 cases of HBsAg, HBeAg, anti- HBc- positive hepatitis B patients, positive HBV DNA in serum was 28 cases, positive rate 93.33% ; 19 cases, positive rate 63. 33% , the difference between the two is significant （ P 〈 0.05 ）. of 30 cases of HBsAg, anti - HBe, anti - HBc hepatitis B patients, positive HBVDNA in serum was eight cases with postive rate 26. 67% ; positive HBVcccDNA in liver tissne was five cases with positive rate 16.67% , the difference between the two was not significant （P 〉0.05） of 10 cases of patients with fatty liver,serum HBV DNA was negative. Conclusions （ 1 ） HBVDNA occured in serum of positive HBeAg was not necesarily to have virus replication ; （2） negative HBeAg or anti HBe was not necesqrily to deny virus replication ; （3） to determine whether the vivus is replicated or not, it showld be confirmed by detection of HBV cccDNA in liver fissue.

关 键 词：肝组织 乙肝病毒共价闭合环状DNA 荧光定量聚合酶链反应 

分 类 号：R512.6[医药卫生—内科学] R512.62[医药卫生—临床医学]