荧光定量聚合酶链反应在巨细胞病毒性肝炎中的应用  

By Fluorescence Quantitative Polymerase Chain Reaction CMV Viral Hepatitis in the Application

作　　者：李宏波[1] 李沛[1] 张永乐[1] 徐爱芳[1] 

机构地区：[1]浙江省杭州市第六人民医院,310014

出　　处：《医学研究杂志》2008年第3期125-126,共2页Journal of Medical Research

摘　　要：目的探讨荧光定量聚合酶链反应(FQ-PCR)在巨细胞病毒性肝炎中的应用价值。方法选50例血清HCMV-IgM阳性的肝炎患者和25例健康人做对照组分别进行血清FQ-PCR法检测其HCMVDNA的含量对比其检出率以及与肝功能的关系。结果50例血清HCMV-IgM阳性的肝炎患者血清HCMVDNA>103拷贝/毫升者35例,HCMVDNA阳性组要较HCM-VDNA阴性组的血清总胆红素(TBiL)谷丙转氨酶(ALT)谷草转氨酶(AST)要高,经统计学处理P<0.05,差异有统计学意义。25例健康对照组HCMV-IgM和HCMVDNA均阴性。结论FQ-PCR在巨细胞病毒性肝炎感染中具有早期诊断结果可靠等特点,通过HCMVDNA含量的变化指导临床、评价药物疗效具有一定的临床应用价值。Objective To investigate the fluorescence quantitative polymerase chain reaction （FQ -PCR ） in the giant cell viral hepatitis in value. Methods 50 cases of serum IgM HCMV - positive patients with hepatitis and 25 cases of healthy people to do the control group respectively serum FQ - PCR detection of its content HCMVDNA its detection rate compared with the liver function and the relationship. Results Of 50 cases of serum IgM HCMV - positive patients with hepatitis HCMVDNA＆gt ; 103Copies/ml those 35 cases, HCMVDNA positive to the negative group than HCMVDNA serum total bilirubin （TBiL） alanine aminotransferase （ALT） aspartate aminotransferase （AST） to the high statistics P ＆It;0.05 the difference was significant. 25 cases of healthy control group and HCMVDNA HCMV - IgM were negative. Conclusions FQ - PCR in cytomegalovirus infection with hepatitis reliable early diagnosis of the characteristics through HCMVDNA content of the clinical evaluation of drug efficacy has certain clinical value.

关 键 词：荧光定量聚合酶链反应 巨细胞病毒 肝炎 

分 类 号：R512.62[医药卫生—内科学] R725.126[医药卫生—临床医学]