微乳毛细管电动色谱分析窗口的研究  

Study on the Migration Window in Microemulsion Capillary Electrokinetic Chromatography

作　　者：李楠 尹昌娜 丁绍东 汪云 曹玉华 

机构地区：[1]江南大学化学与材料工程学院,江苏无锡214122

出　　处：《分析测试学报》2008年第3期256-259,共4页Journal of Instrumental Analysis

基　　金：中国科学院长春应用化学研究所电分析化学国家重点实验室基金资助项目

摘　　要：研究了微乳毛细管电动色谱体系中,表面活性剂、助表面活性剂、微乳液的内相、有机改性剂等微乳组成对分析窗口的影响。以甲醇为电渗流标记物测定电渗流时间,以强疏水性化合物菲为标记物测定微乳液滴(假固定相)的迁移时间,分析时间窗口的变化。最佳条件下,菲的迁移时间和峰面积的相对标准偏差小于1.6%和3.0%(n=6),体系稳定,重复性良好。根据时间窗口的变化规律对其进行适当调节,成功实现了5种水溶性和2种脂溶性维生素的分离,并与毛细管区带电泳及胶束毛细管电动色谱进行了比较。In the system of microemulsion electrokinetic capillary chromatography, the effects of mi- croemulsion compositions on migration window, including the surfactant, co-surfactant, core phases and organic modifiers were studied. Taking methanol as the electroosmotic flow marker and phenan-threne as the microemulsion drop marker, the migration window was analyzed. Under the optimum condition, this proposed method demonstrated good long-term stability and reproducibility with RSD of less than 1.6% and 3.0% for both migration time and peak area of phenanthrene （n = 6）. By adjusting the migration window of microemulsion capillary electrokinetic chromatography according to the experimental results, five kinds of water-soluble vitamins and two kinds of fat-soluble vitamins were successfully separated. Microemulsion capillary electrokinetic chromatography was compared with capillary zone electrophoresis and micellar electrokinetic chromatography in the paper.

关 键 词：微乳液 毛细管电动色谱 时间窗口 维生素 

分 类 号：O657.8[理学—分析化学]