脑室内注射链脲佐菌素大鼠海马神经元和突触的超微结构异常  

Ultrastructural abnormalities of neuron and synapse of hippocampus in the rats treated with intracerebroventricular injection of streptozotocin

作　　者：岳志霞[1] 崔艳君[1] 王蓬文[1] 田金洲[2] 王蓉[3] 盛树力[3] 

机构地区：[1]首都医科大学基础医学院病理学教研室,北京100069 [2]北京中医药大学东直门医院,北京100700 [3]教育部神经变性病学重点实验室,北京100053

出　　处：《电子显微学报》2008年第1期67-72,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家重点基础研究发展计划973项目(No.2003CB517104)

摘　　要：目的:观察侧脑室内重复注射小剂量链脲佐菌素(streptozocin,STZ)阿尔茨海默氏病(alzheimer disease,AD)模型大鼠的学习记忆和海马神经元的超微结构改变。方法:采用Morris水迷宫进行行为学测试;取海马CA1区组织制成超薄切片,透射电镜观察。结果:模型组大鼠Morris水迷宫平均游泳时间及平均游泳距离明显延长。海马CA1区神经元出现程度不等的退行性变。细胞核形态不规则,部分核内染色质浓集;胞浆内脂褐素颗粒增多,高尔基囊泡扩张,严重者出现早期细胞凋亡改变。神经毡内可见有髓鞘轴突胞质内脂褐素颗粒明显增多,突触结构异常,突触小泡聚集增多,分布无序。突触后膜胞质面上的致密斑不规则增厚,或断断续续不完整。结论:侧脑室内注射STZ可引起大鼠海马神经元和突触的超微结构病变,导致大鼠学习记忆能力减退。Objective： To observe the state of learning and memory and ultrastructural changes of hippocampus of the rats which were intracerebroventricularly injected with streptozotocin （STZ）. Methods：After the establishment of AD model, the state of learning and memory of the rats was surveyed by Morris water maze test, and the ultra-structure of hippocampus was observed by using thin-section transmission electron microscopy. Results：The swimming time and swimming distance in Morris water maze test were obviously increased for the rats treated with STZ. The ultrastructural abnormalities of neuron of hippocampus including condensation and margination of chromatin of nuclear, increasing deposition of lipofuscin granules, dilatation of Golgi, cisternae and vesicles in cytoplasm of perikaryon were present. In neuropil, the dysmorphic synapses and axoplasmic degeneration were observed. A great number of small clear synaptic vesicles have aggregated in the synapses and irregular thickening and interrupted macula adherence of postsynaptic membrane could be seen obviously. Conclusion： ICV injection of STZ can injure the hippocampus cause ultrastructural abnormalities of neurons and synapses and cognitive impairment in the rats.

关 键 词：阿尔茨海默病 链尿佐菌素 海马 神经元 突触 超微结构 

分 类 号：R285[医药卫生—中药学]