反相高效液相色谱法测定人胆汁中八种结合胆酸  

SIMULTANEOUS DETERMINATION OF EIGHT KINDS OF CONJUNCT BILE ACIDS IN HUMAN BILE BY R HPLC

作　　者：戴智勇 谭桂山 钱康年 陈萱 

出　　处：《湖南医科大学学报》1997年第4期360-362,共3页Bulletin of Hunan Medical University

摘　　要：采用ＨＰＬＣ法测定人胆汁中八种结合胆酸，色谱柱为ＹＷＧ－Ｃ１８（３μｍ），柱温３０℃，流动相为甲醇／水（６５／３３５，Ｖ／Ｖ，ｐＨ３．０），检测波长２１０ｎｍ。各结合胆酸在５０～１０００μｇｍｌ－１浓度范围呈良好的线性关系，回收率在９１．２％～１０８．６％之间。３０例胆囊结石和２０例无结石病人胆汁样本测定结果显示，二者在胆汁酸的构成上存在差异。A method for the simultaneous determination of eight kinds of conjunct bile acids in human bile was developed by HPLC. They were separated on a YWG-C18 (3μm) column at 30℃, with methanol/water (65/35, V/V, pH3.0) as mobile phase, and detection wavelength at UV 210 nm. The linear ranges were 50～1000μm·ml -1 , the recoveries were 91.2%～108.6%. The biles of 30 cases with cholelithiasis cholecystolithiasis and 20 cases without gallstone were detected by HPLC. The results showed that the constitution of bile acids was different between patients with cholelithiasis cholecystolithiasis and patients without gallstone.

关 键 词：高效液相色谱 结合胆酸 胆汁 胆结石 

分 类 号：R446.1[医药卫生—诊断学] R657.42[医药卫生—临床医学]