苦参碱诱导K562细胞向红系分化伴随凋亡  

Matrine-induced erythroid differentiation of K562 cells is associated with activation of the apoptotic pathway

作　　者：张翠梅[1] 高建慧[1] 李德乐[1] 李璟[1] 施玉麒[1] 林骏[2] 罗深秋[2] 

机构地区：[1]南方医科大学附属中山市博爱医院儿保科,广东中山528403 [2]南方医科大学基础医学院细胞生物教研室,广东广州510515

出　　处：《南方医科大学学报》2008年第3期478-480,共3页Journal of Southern Medical University

摘　　要：目的体外研究苦参碱诱导K562细胞向红系分化伴随的细胞凋亡改变。方法体外培养K562细胞,采用不同浓度的苦参碱诱导K562细胞4d,分别采用倒置显微镜、透射电子显微镜观察诱导前后细胞的形态学和超微结构改变,进一步采用免疫细胞化学方法检测细胞周期调控蛋白p27kip1表达变化。结果与阴性对照组K562细胞相比,0.10g/L浓度的苦参碱诱导后K562细胞形态学和超微结构均显示有凋亡特征,同时细胞内p27kip1蛋白表达明显增加。结论苦参碱在体外诱导K562细胞向红系分化伴随凋亡,可能与p27kip1蛋白表达增加相关。Objective To observe matrine-induced erythroid differentiation of K562 cells in relation to activation of the apoptotic pathway in vitro. Methods K562 cells were cultured in the presence or absence ofmatrine at different concentrations for 4 days, and the morphological and ultramicrostructural changes of the cells were observed using inverted microscopy and transmission electron microscopy, respectively. The expression of apoptosis-related protein p27kipl was detected by immtmocytochemistry. Results Compared to untreated K562 cells, the cells treated with matrine at 0.10 g/L exhibited apoptostic characteristics in the cellular morphology and ultramicrostructure, with the expression of p27kipl protein upregulated in a concentration- and time-dependent manner. Conclusion Matrine-induced erythroid differentiation of K562 cells is associated with cell apoptosis, and upregulation ofp27kipl protein expression may play a crucial role in the process of apoptosis.

关 键 词：苦参碱 K562细胞 细胞分化 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]