紫外分光光度计法与酶标仪微量法测定酚氧化酶蛋白含量及活力的比较  

The comparison of colorimetry and microtitration methods for defermining the protein content and activity of phenoloxidase in insect hemolymph test

作　　者：肖婷[1] 刘守柱[1] 薛超彬[1] 罗万春[1] 

机构地区：[1]山东农业大学农药毒理与应用技术省级重点实验室,山东泰安271018

出　　处：《昆虫知识》2008年第2期306-309,共4页Entomological Knowledge

基　　金：国家自然科学基金资助项目(30571237)

摘　　要：分别采用紫外分光光度计比色法与酶标仪微量法测定菜青虫Pieris rapae(L.)体内酚氧化酶蛋白含量和活力,以水杨醛缩对硝基苯胺为抑制剂,对2种方法的结果进行比较。结果表明,微量法和比色法在检测酚氧化酶(PO)蛋白含量、酶活力和抑制剂对PO抑制作用时结果无显著性差异。因此,微量法可以替代比色法,使用试剂量大大降低,重复性好,操作简单、快捷。Protein content and activity of phenoloxidase（PO） were assayed using microtitration and colorimetry, and the inhibitory effects of salicyhdene-4-nitroaniline on PO activity were also compared by these two methods. The results showed that there were no remarkable differences between these two methods in measurement of PO protein content and activity, and effects of inhibitor （salicylidene-4-nitroaniline） on PO activity. We conclude that microtitration assay is as good as colorimetry, and is even better because it is simple and convenient in operation, and more importantly it needs less amount of reagents.

关 键 词：紫外分光光度计比色法 酶标仪微量法 酚氧化酶 抑制剂 水杨醛缩对硝基苯胺 

分 类 号：Q55-3[生物学—生物化学]