心肌缺血和有氧运动训练诱导VEGF表达时间规律的实验研究  

Temporal phenomenon of myocardial ischemia and aerobic exercises-induced VEGF expression: an experimental study

作　　者：邱峰[1] 励建安[1] 陆晓[1] 黄澎[1] 吴涛[1] 孟祥博[1] 高晶[1] 刘鹏[1] 

机构地区：[1]南京医科大学第一附属医院康复医学科,南京210029

出　　处：《中国康复医学杂志》2008年第3期193-197,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(30370687)

摘　　要：目的:研究心肌缺血和有氧运动训练诱导血管内皮细胞生长因子(VEGF)表达的时间规律。方法:成功建模的健康成年巴马小型猪24只,体重23.7±4.0kg,随机分为对照组、缺血组和运动组。将可控性水囊梗阻器安装在猪冠状动脉钝缘支处,建立可控性猪心肌缺血模型。对照组不进行缺血刺激和有氧运动训练;缺血组进行单纯缺血刺激,2min/次,2次/d,持续缺血刺激8周;运动组除进行缺血刺激外,每天进行一次平板有氧运动训练,每次30min。每两周取动脉血,采用ELISA法检测血液VEGF。实验终点时采用WesternBlot检测缺血区心肌VEGF。结果:缺血组第2周血液中VEGF的表达量明显高于实验前(P<0.05),随后进入平台期(P>0.05)。运动组中第2-4周血液中VEGF的表达量持续增高(P<0.05),随后达到平台期(P>0.05)。缺血区心肌VEGF表达和血液VEGF表达呈正相关(r=0.826,P<0.01)。结论:心肌缺血和有氧运动训练诱导的血液VEGF增高分别在第2周和第4周达到高峰,然后进入平台期。Objective：To study the temporal phenomenon of myocardial ischemia and aerobic exercises training induced expression of vascular endothelial growth factor（VEGF） in swine. Method：Twenty-four miniature swines were randomly assigned to control group （CG）, ischemia group （IG） and exercises group （EG）. A controllable hydraulic balloon catheter was placed at the first marginal branch of left circumflex coronary artery. A model of controllable myocardial ischemia was established. Subjects in IG and EG received transient coronary occlusion for 2rain repeated twice a day for 8weeks. Subjects in EG received 30 rain aerobic exercises training on treadmill with intermittent high intensity 2rain duration which repeated twice a session. Blood samples were obtained every 2 weeks. The ischemic area of the myocardium was also sampled after the completion of 8-week experiment. VEGF in serum were analyzed by ELISA and VEGF in myocardium was measured by Western Blot. Result： VEGF serum level on week 2 in IG was significantly higher than that in CG （P〈0.05） and then reached plateau （P〉0.05）. EG demonstrated the significant increase of VEGF from baseline to week 4 （P〈0.05）, and then reached the plateau （P〉 0.05）. VEGF in serum was positively correlated that in myocardium （r=0.826, P〈0.01）. Conclusion： Intermittent myocardial ischemia and aerobic exercises training induced VEGF expression reaches peak on week 2 and week 4 respectively, and then reaches plateau.

关 键 词：心肌缺血 有氧运动 血管内皮细胞生长因子 

分 类 号：R87[医药卫生—运动医学]