灵芝多糖对AD大鼠海马组织c-fos基因表达的影响  

EFFECTS OF GANODERMA LUCIDUM POLYSACCHARIDE ON C-FOS EXPRESSION IN THE HIPPOCAMPUS OF ALZHEIMER'S DISEASE MODEL RATS

作　　者：李光[1] 袁华[1] 黎莉[1] 甘胜伟[2] 

机构地区：[1]武汉大学医学院人体解剖学与组织胚胎学系,武汉430071 [2]重庆医科大学人体解剖学系,重庆400038

出　　处：《中国组织化学与细胞化学杂志》2007年第6期727-732,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖北省卫生厅基金资助(301140465)

摘　　要：目的研究灵芝多糖(GLP)对AD大鼠组织c-fos基因表达的影响。方法双侧海马内一次性注射β-淀粉样多肽25-35片段(Aβ25-35)制作大鼠AD模型,24h后治疗组用灵芝多糖给予腹腔注射7d,第8d进行Morris水迷宫检测大鼠空间学习记忆能力变化。结束后采用HE染色检测海马神经元的结构变化,免疫组织化学方法检测c-fos基因的表达以及灵芝多糖对其的影响。结果海马内注射Aβ25-35后海马细胞增生、聚集、核边聚、脆裂。GLP组病变则显著减轻,且c-fos基因表达相对AD模型组明显减少。结论灵芝多糖能明显改善AD模型大鼠低下的空间学习记忆能力,显著降低模型大鼠海马组织c-fos基因的表达,对老年性痴呆大鼠学习记忆能力可能有增强和提高作用。Objective To study the effects of Ganoderma Lucidum Polysaccharides （GLP） on the expression of c-fos in the hippocampus of Alzheimer＇s disease model rats. Methods The hippocampus of rats was injected Aβ25-35 bilaterally to induce AD models. After 24 hours the GLP group were injected with GLP solution for 7 days consecutively. At 8d the Morris water maze to study the learning and memory ability. After all the testing, HE stai- ning and c-fos immunohistochemical methods were employed to test the structural changes of hippocampal tissue and c-fos expression. Results Theneurons in the hippocampus of rats injected with Aβ25-35 showed cell increase, endoplasmic reticulum dilatation, chromatin agglomeration, and astrocyte hypertrophy. The pathological changes were alleviated in the GLP group , and the expression of c-fos was markedly reduced when compared with that of the AD model groups. Conclusion GLP can distinctly improve the learning and memory ability, and significantly reduce the expression of c-fos in AD model rats, which suggests that GLP can be used for the treatment of AD in rats.

关 键 词：阿尔茨海默病 Β-淀粉样多肽 灵芝多糖 c—fos 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]