三种化学诱变剂对产氢细菌Ethanoligenens sp．ZGX4的诱变效应  

MUTAGENESIS OF THREE CHEMICAL MUTAGEN ON HYDROGEN-PRODUCING BACTERIUM ETHANOLIGENENS SP.ZGX4

作　　者：郑国香[1] 任南琪[1] 李永峰[1] 林海龙[1] 

机构地区：[1]哈尔滨工业大学市政环境学院,哈尔滨150090

出　　处：《太阳能学报》2008年第1期61-64,共4页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(30470054);国家重点基础研究973发展计划(G2000026402)

摘　　要：分别采用3种不同化学诱变剂(硫酸二乙酯(DES)、1-甲基-3-硝基-1-亚硝基胍(NTG)和吖啶黄)对产氧细菌Ethanoligenens sp.ZGX4进行了诱变处理。DES法获得4种突变体类型,且出现一种自絮凝很强产氢突变类型;NTG法致死率最高.只得到一种类型产氢突变株;吖啶黄法死亡率最低(为21.8%～23.1%).获得2种突变类型。实验证明,DES法的诱变效应最好。高产氢能力突变株的菌落直径变大、透明,但其他形态特征与对照没有太大差异。A preliminary study on chemical mutagenesis of diethyl sulfate （DES）, 1-methyl-3-nitryl-l-nitrosoguanidine （NTG） and acridine yellow on H2-producing strain Ethanoligenens sp. ZGX4 was discussed and showed different mutagenie effects. Four kinds of mutants were obtained by DES mutagenesis, among which included a kind of H2-producing mutant with stronger auto flocculation capacity; The death rate of NTG was maximum and attained one kind of mutant; The death rate of acridine yellow was 21.8% - 23.1% and 2 kinds of mutant were got. Compared with NTG and acridine yellow, the mutagenesis of DES was the best. The morphological characteristic of high efficient mutants was similar with that of control strain except that colonies diameter became bigger and transparent.

关 键 词：化学诱变 产氢发酵细菌 突变体 

分 类 号：X703[环境科学与工程—环境工程]