抗肿瘤坏死因子单克隆抗体对细菌感染小鼠的治疗效果  

The Curative Effects of Anti-TNF Monoclonal Antibody in E. coli Infected Mice

作　　者：郭小清[1] 王爱霞[1] 陈实平[2] 邱志峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学协和医院,北京100730 [2]中国医学科学院基础医学研究所

出　　处：《中国医学科学院学报》1997年第4期312-314,共3页Acta Academiae Medicinae Sinicae

摘　　要：Objective To evaluate effects of monoclonal antibody (McAb) against TNFa on E. coli infected mice. Methods 30 mice (10 week old, Kunming ) were divided equally into three groups. The first group, as a control, 200 μl saline (N S)/mouse was injected intravenously; Second group (untreated group), only E. coli (10 7 organisms/200 μl N S) were injected intraabdominaly; Third group (treated group), E. coli (10 7 organisms) were injected intra-abdominal and McAb against TNFα 2 mg/kg dissolved in 200 μl N S were injected intravenously. After 24 h, observed the survival rate, compared the serum TNF level in blood and investigated pathology of intestine, lung and liver. Results There was a higher survival rate in treated group, with the serum TNF level lowered significantly, and the untreated mice had severe pathologic changes in viscera. Conclusion Using anti-TNFa McAb was effective in reducing mortality rate in mice after infected with E. coli, but could not prevent the pathologic changes.Objective To evaluate effects of monoclonal antibody (McAb) against TNFa on E. coli infected mice. Methods 30 mice (10 week old, Kunming ) were divided equally into three groups. The first group, as a control, 200 μl saline (N S)/mouse was injected intravenously; Second group (untreated group), only E. coli (10 7 organisms/200 μl N S) were injected intraabdominaly; Third group (treated group), E. coli (10 7 organisms) were injected intra-abdominal and McAb against TNFα 2 mg/kg dissolved in 200 μl N S were injected intravenously. After 24 h, observed the survival rate, compared the serum TNF level in blood and investigated pathology of intestine, lung and liver. Results There was a higher survival rate in treated group, with the serum TNF level lowered significantly, and the untreated mice had severe pathologic changes in viscera. Conclusion Using anti-TNFa McAb was effective in reducing mortality rate in mice after infected with E. coli, but could not prevent the pathologic changes.

关 键 词：肿瘤坏死因子 单克隆抗体 细菌感染 

分 类 号：R515.05[医药卫生—内科学]