8-羟基脱氧鸟嘌呤作为可溶性铬盐职业接触者生物标志物的研究  

A Study of Urinary 8-hydroxydeoxyguanosine(8-OHdG)as A Biomarker for Workers Exposed to Soluble Chromate

作　　者：李桂荣[1] 马衍辉[2] 张济[2] 闫蕾[1] 杜仙梅[1] 王翔[1] 刘岚铮[2] 张宁[2] 刘子宏[1] 贾光[1] 张雷 杨晶 

机构地区：[1]北京大学医学部公共卫生学院劳动卫生与环境卫生学系,北京100083 [2]山东省济南市疾病预防控制中心,山东济南250001 [3]江苏省徐州市卫生监督所,江苏徐州221006

出　　处：《预防医学论坛》2008年第2期97-99,共3页Preventive Medicine Tribune

基　　金：国家自然科学基金委员会资助项目(30571550);科技部职业病防治技术标准研究(编号:2002BA906A63);科技部十一五支撑计划(编号:2006BAI06B02)

摘　　要：[目的]了解职业接触可溶性铬盐人群尿中8-羟基脱氧鸟嘌呤(8-OHdG)含量及其影响因素,探讨其作为职业接触人群生物标志物的可行性。[方法]2006年,选择济南市某铬盐生产企业114名重铬酸钾作业的健康劳动者作为接触组,以无毒物接触史的当地健康农民30名为对照组,测定2组人群尿液中8-OHdG水平与作业环境铬盐浓度,测定接触组外周血淋巴细胞DNA链断裂水平、红细胞与尿液中铬浓度。[结果]尿液中8-OHdG水平接触组为(1240.494±1603.918)μg/L,对照组为(468.871±908.460)μg/L(P<0.01);尿液中8-OHdG水平与空气中个体铬盐暴露水平、红细胞中铬浓度水平、外周血淋巴细胞DNA链断裂水平的相关系数(r)分别为0.333、0.230、0.396(P<0.01);多元线性回归分析显示,铬盐劳动者年龄、接触铬盐的年限、空气中铬盐浓度对尿液中8-OHdG水平影响显著。[结论]尿液中8-OHdG水平可以作为职业接触可溶性铬盐劳动者遗传损伤效应性生物标志物,可望用于职业接触可溶性铬盐劳动者生物监测及健康危险性的评价。To explore biological effective markers, urinary 8-hydroxydeoxyguanosine（8-OHdG） content from occupational population with broad ranges of soluble chromate exposure was investigated. [Methods]A cross-sectional study in the chromate exposed workers employed at a chromate factory in a district of Ji＇nan, Shandong was conducted. The studied population contained 114 workers from different processes of the chromate plants, and 30 farmers in the countryside about one hundred kilometers far away the factory without exposure to chromate as a control group. Personal information was obtained by questionnaire. Urinary 8-hydroxydeoxyguanosine （8-OHdG） contents were determined using high performance liquid chromatography with electrochemical detection. DNA strand breakages in lymphocytes were detected by single-cell gel electrophoresis assay （comet assay）. The air concentration of chromate was determined by individual sampling for 8 h per day as shift work and chromium was assayed by atomic absorption spectrometry. The chromium content in the erythrocytes from peripheral blood and urinary was determined by graphite furnace atomic absorption spectrometry. [Results]The results showed that urinary 8-OHdG content in the exposed group was significantly higher than those in the control group （ P〈0.01）. Correlation analysis showed that there was a significant positive correlation between urinary 8-OHdG content and airborne chromate concentration（ P〈0.01）, and urinary 8-OHdG content correlated positively with the chromium content in the erythrocytes from peripheral blood （ P 〈0.01）,and urinary 8-OHdG content correlated positively with the degree of DNA strand breakage in lymphocytes （ P〈0.01）. By multiple regression analysis, we found that age, chromate exposed duration and airborne chromate concentrations were factors that affect the urinary 8-OHdG concentrations. [Conclusion]Our findings suggest that urinary 8-OHdG contcnt was an effective biomarker for occupational chromate-exposed populations

关 键 词：铬酸盐类 职业暴露 8-羟基脱氧鸟嘌呤 

分 类 号：R122.12[医药卫生—环境卫生学]