庚型肝炎病毒5'非编码区基因分析及基因型的初步划分  

Sequence analysis of 5' non-coding region of hepatitis G viruses and preliminary classification of genotypes

作　　者：陈红松[1,2] 王宇[1,2] 王凤水[1,2] 常锦红[1,2] 钱梅云[1,2] 杜绍财[1,2] 

机构地区：[1]北京医科大学人民医院肝病研究所 [2]常州市第三人民医院

出　　处：《中华实验和临床病毒学杂志》1997年第3期212-215,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家863高科技生物计划领域基金

摘　　要：为了分析我国庚型肝炎病毒（ＨＧＶ）的基因结构特点，对２名个体供血者、２例慢性肝炎患者及２例肝硬化患者的血清标本进行了庚型肝炎病毒基因组５’非编码区２７７个核苷酸的基因扩增、分子克隆和序列分析，并利用基因分析软件处理结果。结果表明，分离出的６株ＨＧＶ５’非编码区基因序列（Ｇ００１～Ｇ００６）之间同源性在９６．２％以上。而与国外报道的３株ＨＧＶ序列比较同源性在８６．９％～９１．６％。通过对６株ＨＧＶ（Ｇ００１～Ｇ００６）及国外报道的３株ＨＧＶ基因序列变异性的比较，将ＨＧＶ基因型初步划分为不同的３个组。结果提示我国ＨＧＶ株５’非编码区序列有较高的同源性，而与国外报道的有较大差异，但不同毒株在此区域均可能形成稳定而相似的二级结构模式。For analyzing the structural characteristics of hepatitis G virus (HGV or GBV-C), 277 nucleic acids of 5' non-coding region of HGV were amplified using PCR from the sera of 2 paid donors, 2 chronic hepatitis patients and 2 patients with liver cirrohsis. The products were cloned and sequenced. The data were analyzed with the computer programs. The results indicated that the gene sequence homologies among 6 HGV isolates (G001～G006) obtained in this study were above 96.2%. The comparison of these 6 isolates and 3 foreign isolates reported previously showed that the sequence homologies were in the range from 86.9% to 91.6%. According to the variability of gene sequences, the preliminary division of genotypes of 6 Chinese HGV isolates (G001～G006) and 3 isolates reported abroad were classified into three distinct groups. The results suggest that the gene sequences of Chinese HGV isolates are highly homologous but have an obvious divergence with the foreign isolates. The 5' untranslated regions of all isolates analyzed probably contain a stable and similar secondary structure.

关 键 词：庚型肝炎病毒 病毒基因 序列分析 庚型肝炎 

分 类 号：R512.630.2[医药卫生—内科学] R373.21[医药卫生—临床医学]