中草药叶下珠不同品种和制剂体外抗乙型肝炎病毒的实验研究  

The inhibitory effect of Chinese herb Phyllanthus on hepatitis B Virus in vitro

作　　者：刘庄[1] 傅希贤[1] 张乃临[1] 张国庆[1] 刘丹[1] 

机构地区：[1]北京医科大学第一医院传染病教研室

出　　处：《中华实验和临床病毒学杂志》1997年第3期282-285,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：以２．２．１５细胞为模型，对不同品种、剂型和添加成份的叶下珠提取物进行了体外抗乙型肝炎病毒（ＨＢＶ）活性的研究。结果显示，七种受试药物体外均有不同程度的抗ＨＢＶ活性。其中以沱茶珍珠草、胶囊１号作用最明显，在浓度为５００ｍｇ／Ｌ时对ＨＢｓＡｇ的抑制率可达１００％，而未显示出明显的细胞毒性。Ｓｏｕｔｈｅｒｎ印迹杂交显示，胶囊１号对细胞内外ＨＢＶＤＮＡ的抑制呈剂量依赖型。还观察到，药物对病毒颗粒产生的抑制不仅在转录前水平，可能对ｍＲＮＡ的翻译水平也有影响。Seven preparations of different species of Chinese herb Phyllarthus with different formulations and additives were studied for their ability to inhibit hepatitis B virus DNA replication, that is the HBsAg and HBeAg expression in a HBV DNA-transfected cell line (2.2.15 cell). The results showed that all the drugs were effectively against HBV replication in vitro. Capsule 1 and Tuo Cha Zhen Zhu Cao were found to be the most potent anti-HBV extracts of those examined. Both extracts decreased HBsAg expression by nearly 100% at the concentration of 500mg/L without toxicity on cell growth. Southern blot showed that Capsule 1 inhibited HBV DNA replication in a dose dependent manner. We observed further, that under similar dosage the drug inhibition to HBsAg and HBeAg expression was lower than that to HBV DNA replication, suggesting that the production of progeny virus particles not only were inhibited at the level of HBV DNA synthesis, but also was affected probably at the level of translation of viral mRNA.

关 键 词：乙型肝炎病毒 2.2.15细胞株 苦味叶下珠 

分 类 号：R373.21[医药卫生—病原生物学]