载有溶菌酶微球的凝胶埋植剂的释药特性  

Controlled release of lysozyme-loaded microspheres in hydrogel implants

作　　者：孙佳丽[1] 林莹[1] 蒋国强[1] 昝佳[1] 丁富新[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《清华大学学报（自然科学版）》2008年第3期395-398,共4页Journal of Tsinghua University(Science and Technology)

基　　金：国家自然科学基金资助项目(20576057);北京市自然科学基金资助项目(2052012)

摘　　要：为实现生物大分子药物的体内长期缓释,将药物制成乳酸/羟基乙酸共聚物(PLGA)的微球,再将微球分散于甲基纤维素反向温敏凝胶骨架中,制成微球-温敏凝胶复合埋植剂。通过体外释放实验,考察复合体系对模型药物溶菌酶的控释效果;通过凝胶色谱和扫描电镜,考察单独的微球以及在复合体系中的微球降解过程,分析凝胶介质对微球降解特性的影响;采用Peppas经验式来描述释放机制。结果表明:在微球-凝胶复合体系中,溶菌酶可平稳释放30 d以上,无突释;药物释放是由载体溶蚀和药物扩散作用共同控制的。Drug-loaded poly （lactic-co-glycolic） acid （PLGA） microspheres were dispersed in methylcellulose reverse thermo-sensitive hydrogel for long-term sustained release of biomacromolecular drugs. The in vitro release properties of the model drug lysozyme in the mixture formulation were examined. The influence of the hydrogel on the degradation of the PLGA was investigated using gel permeation chromatography （GPC） and a scanning electron microscope （SEM）. The mechanisms for drug release from the microspheres in the hydrogel were deduced by Peppas＇ formula. The results show that the mixture formulation exhibits nearly zero-order release of lysozyme for over 30 days without any burst effect. The drug release is controlled by both drug diffusion and material erosion.

关 键 词：埋植剂 PLGA微球 温敏水凝胶 Peppas经验式 

分 类 号：R944[医药卫生—药剂学]