美洲拟鲽抗冻蛋白基因(afp)导入番茄的研究  

The Research on Introducing Flounder Antigreeze Protein Gene(afp)into Tomato

作　　者：黄永芬[1] 汪清胤[1] 付桂荣[1] 赵晓祥[2] 杨志兴 

机构地区：[1]哈尔滨师范大学生物系 [2]黑龙江省应用微生物研究所

出　　处：《生物化学杂志》1997年第4期418-422,共5页

基　　金：国家自然科学基金资助项目(39O7O595)

摘　　要：将整合在Ｔｉ质粒上的ａｆｐ基因作为供体ＤＮＡ，用花粉管通道和子房注射方法导入番茄品种“中蔬四号”中，对Ｄ１、Ｄ２代进行Ｓｏｕｔｈｅｒｎ杂交获得杂交带；田间抗寒性试验表明，在春季平均气温低于正常年份４·４℃条件下，转基因组植株生长势优于对照；致死温度也比对照降低２℃．Antifreeze protein gene(afp)which is integrated into the Ti plasmid pMon322,was introduced into tomato via the pollen tube pathway and ovary injection.The transgenic plants of D 1 and D 2 generation produced afp DNA on Southern blot.The assay of cold resistance in the filed showed that transgenic plants could grow better than control under the average temperature that is 4.4℃ below normal year in earlier spring,meanwhile the fatal temperature dropped 2℃.These results revealed that AFP gene has been integrated into the transgenic plants genome and expressed.

关 键 词：番茄 美洲拟鲽 抗冻蛋白基因(afp) 外源DNA注射 

分 类 号：S641.203.2[农业科学—蔬菜学] S188[农业科学—园艺学]