人外周型苯二氮卓受体及其突变受体cDNA克隆和序列分析  

Cloning of the cDNAs Encoding Human Peripheral Benzodaizepine Receptor and Its Mutant Receptor

作　　者：徐国恒[1] 胡本荣[1] 罗超权[1] 陈汝筑[1] 邱鹏新[1] 

机构地区：[1]中山医科大学药理学系生化和分子生物学系

出　　处：《生物化学杂志》1997年第4期423-427,共5页

摘　　要：用ＲＴ－ＰＣＲ方法扩增并克隆了三种人外周型苯二氮卓受体ＰＢＲｃＤＮＡ，测序表明，４４２ｂｐ片段与文献报道相比缺失８４ｂｐ编码序列，其转录水平高于正常ＰＢＲ．该序列编码一个与ＰＢＲ结构相关但缺失了２８个氨基酸残基的突变受体蛋白．这一异常转录本可能是通过选择性剪接方式转录产生并只存在于中国人肝癌ＢＥＬ７４０２细胞系，表明ＰＢＲ基因表达具有细胞特异性和异质性．Three of the human peripheral benzodiazepine receptor cDNA were detected and cloned via RT PCR method.One of these,a 442 bp amplification product has a 84 bp deletion and its transcript amount was higher than that of the native PBR.This abnormal transcript probably occurs through alternative splicing at transcriptional level,and it presents only in the human hepatoma BEL 7402 cell line.The results suggest that the expression of the PBR gene has cellular specificity and heterogenicity,which probably regulate the cellular function of the PBR at molecular level.The finding of the mutant PBR can provide a molecular and cellular model usefully to study the structure and function of the PBR.

关 键 词：苯二氮卓 受体 基因克隆 突变 CDNA 受体药理学 

分 类 号：R966[医药卫生—药理学]