油桐尺蠖核型多角体病毒多角体蛋白的理化性质及二级结构预测  

Physicochemical Properties and Secondary Structure Prediction of Buzura suppressaria Nuclear Polyhedrosis Virus Polyhedrin

作　　者：陈华[1] 陈东[1] 龚为民[1] 滕脉坤[1] 朱学勇[1] 牛立文[1] 

机构地区：[1]中国科学技术大学生物系结构生物学青年实验室,合肥230026

出　　处：《生物化学杂志》1997年第3期308-311,共4页

摘　　要：由SDS及梯度胶电泳测得油桐尺蠖核型多角体病毒（BsNPV）多角体蛋白天然状态及亚基分子量分别为363kD与31.5kD，从而推断此蛋白为十二聚体，亚基间无二硫键作用．BsNPV多角体蛋白的远紫外圆二色谱显示，它的二级结构含有31．7％的α螺旋，23．8％的β折叠及44．5％的无规卷曲，与二级结构预测结果相符．通过荧光光谱实验推知，BsNPV多角体蛋白的表面疏水性弱，其色氨酸残基位于蛋白疏水核内部．Using SDS-PAGE and gel concentration gradient electrophoresis,It was determined that the molecular weights of Buzura suppressaria nuclear polyhedrosis virus (BsNPV) polyhedrin and its subunit are 363 kD and 31. 5 kD respectively and this protein was composed of twelve subunits without disulfide bonds among the subunits. The circular dichroism (CD) spectrum indicated that the secondary structure of polyhedrin consisted of 31, 7 % α-helix, 23. 8% βsheet,and 44. 5 % random-coil,which was in coincidence with the result of prediction. The fluoresence studies of polyhedrin showed that the surface hydrophobicity of polyhedrin was weak and the tryptophan residues in each subunit were in the internal hydrophobic region.

关 键 词：油桐尺蠖 核型多角体病毒 多角体蛋白 二级结构 

分 类 号：Q965.8[生物学—昆虫学]