NB901的细胞毒作用及对拓扑异构酶Ⅱ活性的影响  

The Cytotoxicity and Effect of NB901 on DNA Cleavage Reaction by Topoisomerase Ⅱ

作　　者：战洪生[1] 李润沼[1] 臧传兵 王纳[1] 

机构地区：[1]北京医科大学天然药物及仿生药物国家重点实验室,北京100083

出　　处：《生物化学杂志》1997年第3期368-370,共3页

摘　　要：The cytotoxicity and effect of NB901 (one of mono-nucleoside derivative)on pBR322 DNA cleavage reaction by topoisotnerase Ⅱ werestudied. The results showed that 50% inhibiting concentration (IC50)of the drug against human hepatoma cell Bel 7402 and lung cancer cell PGT by neutral red colorimetry test in vitro were 7. 32 and 3. 53 μmol/L respectively. The presence of NB901 (5—10μmol/L)markedly stimulated DNA cleavage by 5-fold in the presence of 0. 2 U topoisornerase Ⅱ. It is suggested that topoisomerase Ⅱ be a possible target for NB901 which result in cytotoxicity.The cytotoxicity and effect of NB901 (one of mono-nucleoside derivative)on pBR322 DNA cleavage reaction by topoisotnerase Ⅱ werestudied. The results showed that 50% inhibiting concentration (IC50)of the drug against human hepatoma cell Bel 7402 and lung cancer cell PGT by neutral red colorimetry test in vitro were 7. 32 and 3. 53 μmol/L respectively. The presence of NB901 (5—10μmol/L)markedly stimulated DNA cleavage by 5-fold in the presence of 0. 2 U topoisornerase Ⅱ. It is suggested that topoisomerase Ⅱ be a possible target for NB901 which result in cytotoxicity.

关 键 词：NB901 抗癌药 细胞毒作用 拓扑异构酶Ⅱ 

分 类 号：R979.19[医药卫生—药品] R965[医药卫生—药学]