作用于pNPP的磷酸酶在凝胶中的特异性染色  

A Specific Staining Method for Detecting The p-Nitrophenylphosphatase in Polyacrylamide Gels

作　　者：姚知行[1] 夏英[1] 颜卉君[1] 

机构地区：[1]北京师范大学生物系,北京100875

出　　处：《生物化学杂志》1997年第2期237-239,共3页

摘　　要：A simple, rapid and sensitive staining method for detecting the phosphatase in polyacrylamide gels was developed, in which p-nitrophenyl phosphate (pNPP) was used as substrate.This method was based on the activity of p - nitrophenylphosphatase (pNPPase) to release Pi from pNPP within the polyacrylamide gels which then combines with lead ion to form the lead phosphate and precipitates in the gel as white bands. These bands can be changed to dark brown bands on a transparent background due to formation of PbS by treating with (NH4)2S within the gel.The crude extracts of H22a hepatoma ascites cells and normal mouse liver cells were analyzed by this method and found that there are some difference between the two kinds of cells.A simple, rapid and sensitive staining method for detecting the phosphatase in polyacrylamide gels was developed, in which p-nitrophenyl phosphate (pNPP) was used as substrate.This method was based on the activity of p - nitrophenylphosphatase (pNPPase) to release Pi from pNPP within the polyacrylamide gels which then combines with lead ion to form the lead phosphate and precipitates in the gel as white bands. These bands can be changed to dark brown bands on a transparent background due to formation of PbS by treating with (NH4)2S within the gel.The crude extracts of H22a hepatoma ascites cells and normal mouse liver cells were analyzed by this method and found that there are some difference between the two kinds of cells.

关 键 词：磷酸酶 硝基苯磷酸盐 染色法 

分 类 号：Q555.702[生物学—生物化学] Q－336