重组[B18Ile]人胰岛素的鉴定和特征  

Expression and Activity of Recombinant Human Augmenter of Liver Regeneration

作　　者：刘滨[1] 唐月华[1] 梁镇和[1] 冯佑民[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1997年第4期409-413,共5页

基　　金：国家高技术研究资助

摘　　要：突变体［Ｂ１８Ｉｌｅ］猪胰岛素前体经分离纯化、转肽，得到重组［Ｂ１８Ｉｌｅ］人胰岛素。［Ｂ１８Ｉｌｅ］人胰岛素能结晶，其与受体的结合能力为猪胰岛素的８２％，保留了与猪胰岛素基本相同的体内生物活力。从本文结果和分析表明Ｂ１８Ｖａｌ可能不是胰岛素表现生物功能所必需的。The cDNA of human augmenter of liver regeneration was subcloned onto the downstream of the P RP L promoter of the expression plasmid pBV220. The recombinant plasmid could stabelly express ALR with high efficiency (up to 20% of the total bacterial proteins) in E.coli through thermal induction.The expressed recombinant ALRs could produce a significant increase in the incorporation of 3 H TdR into liver DNA of a 1/3 hepatectomized test animal and also had a potent antihepatitis effect. These results suggest that ALR appears to be an important regulator of liver regeneration and may be used in clinical trial for enhancing liver regeneration in the treatment of hepatic diseases.

关 键 词：胰岛素 结构 功能 重组 鉴定 

分 类 号：Q578.03[生物学—生物化学]