BxPC-3条件培养液对PC12细胞株DA和NE代谢的影响  

Effect of BxPC-3-conditioned medium on the metabolism of DA and NE in PC12 cell line and its mechanism of action

作　　者：李荣[1] 王杰军[1] 吴欣[1] 郭静[1] 王妙苗[1] 王喜[1] 解静[1] 何晓静[1] 

机构地区：[1]中国人民解放军第二军医大学附属长征医院肿瘤科,上海市200070

出　　处：《世界华人消化杂志》2008年第5期484-487,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨BxPC-3条件培养液影响分化的PC12细胞神经递质代谢的机制.方法:用去除IL-6的BxPC-3条件培养液处理分化的PC12细胞,采用流式细胞仪检测各组细胞凋亡率、HPLC检测上清和细胞裂解液中DA、5.HT和NE的含量.在不含血清的条件培养液中加入IL-6(0.01mg/L,0.1mg/L,0.25 mg/L,0.5 mg/L,1mg/L,1.5 mg/L,2 mg/L),分别收集上清和细胞裂解液,采用HPLC检测DA和NE的含量.结果:各组细胞凋亡率之间差别无统计学意义,条件培养液中加入IL-6抗体后,上清中DA和NE的含量明显增加,而细胞内DA和NE代谢差异无统计学意义,在加入不同剂量IL-6后,随着浓度的增加,DA和NE的代谢近似呈现剂量依赖性降低,当IL-6浓度为1mg/L时DA和NE的变化有统计学意义.结论:BxPC-3条件培养液主要通过IL-6影响分化的PC12神经递质代谢.AIM： To study the effect of BxPC-3-conditioned medium on the metabolism of DA and NE in PC12 cells and its mechanism of action. METHODS： PC12 cells were treated with BxPC- 3-conditioned medium without IL-6. The rate of apoptosis of PC12 cells was detected by flow cytometry. DA, 5-HT and NE in the medium were measured by HPLC. IL-6 （0.01 mg/L, 0.1 mg/L, 0.25 mg/L, 0.5 mg/L, 1 mg/L, 1.5 mg/L, 2 mg/ L） was added into the medium deprived of serum, then the DA, 5-HT and NE in the medium were measured. RESULTS： There was no significant difference in the rate of apoptosis of PC12 cells in each group. After anti-IL-6 was added into the conditioned medium, the quantity of DA and NE in the medium was increased. The concentration of IL-6 was increased. DA and NE were metabolized in a dose-dependent manner. Addition of 1mg/L IL-6 significantly decreased DA and NE. CONCLUSION： BxPC-3-conditioned medium has certain effects on the metabolism of DA and NE of PC12 cells by adding IL-6.

关 键 词：条件培养液 白介素6 PC12细胞 多巴胺 去甲肾上腺素 代谢 机制 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]