胆道梗阻再通术后肾脏AQP3的变化及其意义  

Change of renal aquaporin 3 expression in rats after release of obstructive jaundice and its significance

作　　者：李云飞[1] 刘金钢[2] 

机构地区：[1]暨南大学附属第一医院急诊科,广东省广州市510632 [2]中国医科大学盛京医院第二胆道微创外科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2008年第5期517-521,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨大鼠胆道梗阻再通术后肾脏水通道蛋白3(AQP3)的变化及其意义.方法:50只大鼠,任选10只为对照组,其余为实验组,实验组先建梗阻性黄疸(obstructive jaundice,OJ)模型,7 d后解除梗阻;按取材时间不同随机分为5组:OJ0h、OJ24h、OJ72 h、OJ1wk和NC.测定血生化指标.Western blot法检测肾脏AQP3水平.结果:随梗阻解除时间延长血清总胆红素(TBIL)和谷丙转氨酶(ALT)逐渐下降(TBIL:93.26±1.32 vs 63.31±1.85,30.78±1.40,5.04±0.24,P<0.05:ALT:70.95±1.22 vs 69.96±0.82,30.74±1.52,11.84±1.12,P<0.05):OJ0 h与NC相比尿素氮(BUN)、血肌酐(Cr)无明显变化(P>0.05),AQP3表达降低;OJ24h BUN、Cr下降,AQP3表达进一步降低.OJ 72 h与OJ 24 h相比BUN、Cr无明显变化,AQP3表达增强,但仍弱于NC(P<0.05);到第7天,BUN、Cr和AQP3均与NC无明显差别.结论:大鼠胆道梗阻解除后短期内因肾功能受损加重,AQP3表达下降后随肾功好转表达增强,其灵敏性和特异度均高于BUN和Cr.AIM： To study the change of renal aquaporin 3 （AQP3） expression in rats after release of obstructive jaundice in biliary tract and its significance. METHODS： Fifty Wistar rats were divided into control group （n= 10） and experimental group （n = 40）. A model of experimental obstructive jaundice was established. Seven days after release of obstructive jaundice, serum biochemical parameters were detected. Rats in the experimental group were randomly divided into five subgroups： OJ 0 h, OJ 24 h, OJ 72 h, OJ 1 wk and non-operated control （NC）. Biochemical parameters in venous blood were detected and the expression level of renal AQP3 was determined by Western blot. RESULTS： The TBIL and ALT gradually decreased after release of obstructive jaundice in biliary tract （TBIL： 93.26 ± 1.32 vs 63.31 ± 1.85, 30.78 ± 1.40, 5.04 ± 0.24, P 〈 0.05; ALT： 70.95 ±1.22 vs 69.96 ± 0.82, 30.74 ± 1.52, 11.84 ± 1.12, P 〈 0.05）. There was no significant difference in levels of BUN and Cr between the OJ 0 h and NC subgroups, but the expression level of AQP3 was lower in the OJ 0 h subgroup than in the NC subgroup. Both BUN and Cr were significantly decreased 24 h after operation and the expression of AQP3 was further decreased. The levels of BUN and Cr did not markedly change between the OJ 24 h and OJ 72 h subgroups. The expression level of AQP3 was slightly higher, but still lower in the OJ 24 h subgroup than in the NC subgroup （P 〈 0.05）. The expression levels of BUN, Cr and AQP3 were not significantly different between the two groups 7 days after operation. CONCLUSION： The renal function is further damaged after release of obstructive jaundice in biliary tract. The expression level of AQP3 becomes higher when renal function improves. The sensitivity and specificity of AQP3 are higher than those of BUN and Cr.

关 键 词：梗阻性黄疽 肾功能衰竭 水通道蛋白3 尿素氮 血肌酐 

分 类 号：R657.4[医药卫生—外科学]