PGP9.5和NPY肽能神经在成人睾丸组织中的分布  

Distribution of PGP9.5 and NPY immunoreactive peptidergic nerves in adult human testes

作　　者：宗益平[1] 顾民[1] 龚永光[1] 陈琛君[1] 

机构地区：[1]南京医科大学第一附属医院泌尿外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第3期327-329,334,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30400161)

摘　　要：目的:研究蛋白基因产物9.5(proteingeneproduct9.5,PGP9.5)肽能神经和神经肽Y(neuropeptideY,NPY)肽能神经在成人睾丸组织中的分布情况。方法:成年男性睾丸标本4例,将其制成5!m厚冰冻切片,运用ABC免疫组织化学方法检测睾丸组织中PGP9.5与NPY肽能神经纤维的分布。结果:PGP9.5肽能神经纤维主要分布于睾丸间质中,其与曲细精管及Leydig细胞存在较为紧密的联系;NPY肽能神经纤维主要分布于睾丸血管周围,生精小管管周也可见部分分布,NPY神经纤维分布密度低于PGP9.5神经纤维。结论:人睾丸组织内分布着PGP9.5和NPY肽能神经纤维,精索神经通过这些神经纤维实现其对精子发生过程的调控。Objective：To study the distribution patterns of PGP9.5 and NPY immunoreactive （IR） peptidergic nerves in adult human testes. Methods：Four testes from adult males were processed for 5 μm thick frozen sections. Antibodies against protein gene product（PGP） 9.5 and neuropeptide Y（NPY） were used for immunohistochemical detection of peptidergic nerves in the human testes. Results： PGP9.5-IR fibers were mainly distributed in the testicular interstitium,ln close association with seminiferous tubules and Leydig cell nests. The majority of the NPY-IR nerves were observed around intratesticular blood vessels,but the walls of the seminiferous tubules were also innervated. The density of PGP9.5 immunoreactive nerve was stronger than that of NPY. Conclusion：The presence of peptidergic nerves in the human testes provides anatomic evidence for involvement of the spermatic nerves in spermatogenesis regulation.

关 键 词：睾丸 PGP9.5 NPY 

分 类 号：Q593.2[生物学—生物化学]