NTG和UV复合诱变选育紫杉醇高产菌株的研究  

Screening of high taxol producing fungi by NTG combining with UV mutagenesis

作　　者：赵凯[1] 王颖[1] 王旋[1] 孙宇石[1] 平文祥[1] 周东坡[1] 

机构地区：[1]黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2008年第1期74-77,共4页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(30570025);黑龙江省"十五"重大攻关项目(GA02C101);黑龙江省教育厅海外学人科研资助项目(1152HZ06);哈尔滨市青年科学基金项目(2005AFQXJ063);黑龙江大学杰出青年基金资助项目

摘　　要：对紫杉醇产生菌HD1-3的孢子分别进行了紫外线照射(UV)、亚硝基胍(NTG)和UV+NTG复合诱变处理,筛选制霉菌素抗性突变株。试验所确定的Nodulisporium sylviforme紫杉醇产生菌HD1-3孢子诱变的适宜条件为:将孢子悬液经过3.0mg·mL-1 NTG、处理20min后,在电磁搅拌下,用紫外灯(30w,距离30cm)照射处理45s。共筛选出17株正突变株。经发酵筛选试验,获得了一株遗传性状稳定、高产紫杉醇的诱变菌株——UN25-3,其紫杉醇产量从出发菌株的(232.73±4.61)μg·L-1提高至(327.14±7.63)μg·L-1。In order to obtain resistant mutants to nystatin, ultraviolet radiation, NTG and UV ＋ NTG were used to mutagenize the spores of taxol - producing fungus HD1-3, respectively. The spore suspension was treated with 3.0 mg ·mL^- 1 NTG for 20 min, followed by UV irradiation （ 30w, 30cm distance ） for 45 s under magnetic stirring. Seventeen strains were found to be positive mutants with high taxol when screened on plate containing 95 μg· L^-1 nystatin. After further screening experiments on fermentation, a mutant -UN25-3 which was able to produce taxol with high yield and could be stably passed on genetics was eventually found, it＇ s ability to produce taxol was improved from （232.73±4. 61） μg· L^-1（strain HD1-3）to （327.14±7.63） μg· L^-1（strain UN25-3）.

关 键 词：紫杉醇 内生真菌 诱变 选育 

分 类 号：Q94[生物学—植物学]