无机抗菌剂对自酸蚀处理剂全身毒性和细胞毒性影响的研究  

Influence of inorganic antibacterial agents on the systemic toxicity and cytotoxicity of a self-etching primer

作　　者：方明 陈吉华 柴枫 贾敏[3] HILDEBRAND Hartmut F 

机构地区：[1]第四军医大学口腔医院修复科,陕西西安710032 [2]Groupe de Recherche sur les Biomateriaux,Laboratoire de Biophysique UPRES EA 1049,Facuh6 de M6decine,Universite de Lille 2,F-59045 Lille Cedex,France [3]第四军医大学药学系药理教研室,陕西西安710032

出　　处：《华西口腔医学杂志》2008年第1期31-35,共5页West China Journal of Stomatology

摘　　要：目的测试无机抗菌剂的添加对新型实验自酸蚀处理剂(ESP)全身毒性和细胞毒性的影响。方法将6种无机抗菌剂分别加入ESP中,进行经口途径大鼠短期全身毒性实验。并采用受试材料与NIH3T3细胞直接接触培养的方法,观测其对细胞增殖、活力和形态的影响。结果抗菌剂添加比为5.0%时,为期2周的大鼠全身毒性实验中未发现临床毒性体征,各组间大鼠体重相对增长率无显著差异。经含质量浓度为0.5%不同抗菌剂的处理剂处理固化后的各实验组细胞活力比率和细胞增殖率与ESP空白对照组相比无显著差异。仅样本覆盖区及样本边缘邻近区域细胞可观察到毒性表现,远离样本覆盖区的细胞密度未受明显影响。结论以适当的质量浓度添加受试的无机抗菌剂对受试ESP的全身毒性和细胞毒性无显著影响。Objective To evaluate the influence of incorporating inorganic antibacterial agents on the systemic toxicity and cytotoxicity of an experimental self-etching primer（ESP）. Methods Six kinds of inorganic agents were incorporated respectively into the primer. Systemic toxicity in vivo tests in rats and direct contact in vitro cytotoxicity tests with NIH fibroblasts were conducted. Results Systemic toxicity tests revealed neither toxic manifestations nor significant differences in body weight gain between control and other groups. There were no significant differences between experimental groups and empty control on cell vitality and cell proliferation rates. Toxicity was only observed in areas beneath the specimens and/or in the direct vicinity of the specimen edge. There was no influence on the cell density over the limit of specimens. Conclusion The incorporation of tested inorganic antibacterial agents with a proper concentration had no significant influence on the systemic toxicity and cytotoxicity of the tested selfetching primer.

关 键 词：无机抗菌剂 自酸蚀处理剂 全身毒性 细胞毒性 

分 类 号：R114[医药卫生—卫生毒理学]