小麦TaMBD4基因的克隆、结构及表达的初步分析  

Preliminary Study on Cloning,Structure and Expression of TaMBD4 Gene in Wheat

作　　者：孟凡荣[1] 刘昊英[1] 司志飞[1] 尹钧[2] 李永春[2] 

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]河南农业大学国家小麦工程技术研究中心,郑州450002

出　　处：《植物生理学通讯》2008年第1期20-24,共5页Plant Physiology Communications

基　　金：国家自然科学基金项目(30300195)。

摘　　要：以拟南芥MBD基因的EST为基础,采用电子克隆并结合RT-PCR方法分离克隆了包含开放阅读框的小麦甲基结合域蛋白基因TaMBD4。序列分析显示,TaMBD4蛋白有典型的甲基结合域。组织表达特性分析表明,TaMBD4在干种子和胚乳中的表达量高于其它组织。TaMBD4的cDNA和基因组DNA比较分析显示,此基因包括1个内含子,进一步分析表明这个内含子为2个GGCAGT序列的串联重复,推测该内含子可能与TaMBD4基因的转录后调控相关。In order to provide an insight into molecular characteristics and putative epigenetic functions of MBD in wheat, a gene with complete open reading frame （ORF） designated as TaMBD4 was identified in wheat by in silico cloning approach and its fragment was cloned by RT-PCR. Amino acid sequence analysis showed that TaMBD4 contained a conserved methyl-CpG binding domain, which might play a putative role in epigenetic mechanism. Spatial expression analysis showed that the expression level of TaMBD4 was higher in dry seeds and endosperm than that in other tissues tested. The compartive analysis between cDNA and genomic DNA of the gene showed that a short intron was included. Interestingly, we found that the intron was composed of 2 tandem simple sequence repeats, which might be involved in the posttranscriptional regulation of the TaMBD4.

关 键 词：DNA甲基化 小麦 TaMBD4 克隆 基因结构和表达 

分 类 号：Q943.2[生物学—植物学] S512.1[农业科学—作物学]