香果树组织培养过程中遗传变异的RAPD分析  

Analysis of Genetic Variation in Emmenopterys henryi Oliv.in vitro by RAPD Fingerprinting Method

作　　者：熊丹[2] 谢伟[2] 陈发菊[1,2] 梁宏伟[2] 王玉兵[2] 

机构地区：[1]湖北民族学院,湖北省生物资源保护与利用重点实验室,湖北恩施445000 [2]三峡大学生物技术研究中心,湖北省植物天然产物重点实验室,湖北宜昌443002

出　　处：《植物生理学通讯》2008年第1期37-41,共5页Plant Physiology Communications

基　　金：湖北省生物资源保护与利用重点实验室开放基金(2007010);三峡大学重大项目(2002C03);宜昌市科技重点攻关项目(A06209)。

摘　　要：用RAPD分子标记方法,从DNA水平上分析野生型香果树以及通过器官发生途径和体细胞胚胎发生途径得到的香果树再生植株以及体细胞胚胎发生过程中不同继代次数的培养物之间的遗传变异。筛选了100个随机引物,其中有75条随机引物能够扩增出条带,从中选取11个引物进行PCR扩增的结果显示:香果树体细胞胚胎无性系中有RAPD多态性位点,在胚性愈伤组织中也检测到少数RAPD变异位点。表明RAPD分子标记方法可以鉴定香果树组织培养过程中的遗传变异。Randomly amplified polymorphic DNA （RAPD） analysis was used as a tool to assess the genetic variation of organogenesis and somatic embryogenesis-derived plants and embryonic callus of subcultures of Emmenopterys henryi. Eleven random decamer primers were successfully used to analyze genomic DNA from the parental field-grown plants, organogenesis and somatic embryogenesis-dedved plants material and embryonic callus of subcultures. A total of 89 scoreable fragments were amplified with an average of 8.09 bands per primer per lane. The results indicated that RAPD markers could be applied successfully as a rapid, simple and cheap tool for the establishment of genetic variation between donor parental field grown plants and micropropagated material.

关 键 词：香果树 RAPD分析 体细胞胚胎发生 遗传变异 

分 类 号：Q943[生物学—植物学] S792.99[农业科学—林木遗传育种]