机械刺激诱导的烟草悬浮培养细胞耐热性及其与过氧化氢的关系  

Mechanical Stimulation-induced Heat Tolerance of Suspension Culture Cells in Tobacco(Nicotiana tobacum L.)and Its Relation to H_2O_2

作　　者：李忠光[1] 龚明[1] 

机构地区：[1]云南师范大学生命科学学院,昆明650092

出　　处：《植物生理学通讯》2008年第1期42-44,共3页Plant Physiology Communications

基　　金：国家自然科学基金(30460016);云南省自然科学基金(2006C0030M);云南省教育厅基金(06Y117B)。

摘　　要：机械刺激可诱发烟草悬浮培养细胞中H_2O_2的积累,提高烟草悬浮培养细胞在高温胁迫下的存活率和再生能力,缓解高温胁迫下的细胞活力丧失和膜伤害,外源H_2O_2预处理也可提高烟草悬浮细胞的耐热性。这些暗示H_2O_2作为信号分子可触发机械刺激诱导的烟草悬浮细胞耐热性形成。Mechanical stimulation （MS） induced H2O2 accumulation in tobacco suspension culture cells, increased survival percentage and regrowth of tobacco suspension cells under high temperature at 43 ℃, and alleviated the loss of cell vitality and membrane damage. In addition, exogenous H2O2 pretreatment also significantly enhanced heat tolerance in tobacco suspension cells. These results suggested that H2O2 could act as a signaling molecule to induce the formation of MS-induced heat tolerance in tobacco suspension cells.

关 键 词：烟草悬浮培养细胞 机械刺激 耐热性 H2O2 

分 类 号：Q943.1[生物学—植物学]