大赖草总DNA转化小麦的RAPD分析  

RAPD Analysis of Transgenic Wheat(Triticum aestivum L.)with Total DNA of Leymus racemosus(Lam.)Tzvel.

作　　者：赵民安[1] 刘敏[1] 曹玉锦[1] 

机构地区：[1]中国科学院新疆理化技术研究所,乌鲁木齐830011

出　　处：《植物生理学通讯》2008年第1期55-58,共4页Plant Physiology Communications

基　　金：新疆维吾尔自治区专利实施项目(2004201072916)。

摘　　要：用160个随机引物对不同性状转基因小麦、受体‘春麦761’和大赖草等进行随机扩增多态性DNA(RAPD)分析,筛选出5个重复性好和多态性高的引物。转基因小麦的扩增图谱中出现供体大赖草的特异性条带,简单遗传相似系数和聚类的分析结果均表明,大赖草基因片段已整合到转化的小麦基因组中。Genetic diversity of transgenic wheat with different phenotypes, ‘spring wheat 761＇ and Leymus racemosus was analyzed by random amplified polymorphic DNA （RAPD） with 160 arbitrary oligonucleotide primers. Five primers with good repetition and high polymorphism were screened. There were several special bands the same as the donor in the transgenic wheat. The genetic similarity coefficient and clustering analysis also confirmed that the gene fragments of L. racemosus had been integrated into the genome of transgenic wheat.

关 键 词：大赖草 RAPD 转基因小麦 

分 类 号：S512.1[农业科学—作物学] TQ464.2[化学工程—制药化工]