海桐花苯丙氨酸解氨酶的基因克隆与序列分析  

Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase cDNA from Pittosporum tobira

作　　者：许锋[1] 陈柳吉[1] 蔡荣[1] 杜何为[2] 程水源[1] 

机构地区：[1]长江大学园艺园林学院,荆州434025 [2]长江大学生命科学学院,荆州434025

出　　处：《西北农业学报》2008年第2期218-224,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：Programfor New Century Excellent Talents in University(NCET-04-0746);the Region Technology Development Programof Chinese Ministry of Education(02095);the Natural Science Foundation of Hubei Province(2002AB094);the Youth Talent Foundation of Hubei Province(2003AB014);the Educational Office Key Research Programof Hubei Province(Z200627002)

摘　　要：从海桐中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段,并命名为PitPAL,GenBank登录号为AY747678。PitPAL长866个bp,编码289个氨基酸。通过核苷酸和蛋白质序列多重比较发现PitPAL与其他植物的PAL基因高度同源。PitPAL编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树表明PitPAL与乔木类植物(如夹竹桃、山茶)的PAL基因聚类关系最近。PitPAL基因的克隆为利用基因工程技术来调控海桐花青苷的合成代谢、以及海桐花的颜色调控提供了基因资源。A phenylalanine ammonia-lyase （PAL） gene （designated as PitPAL） was cloned from Pittosporum tobira. The PitPAL cDNA fragment was 866bp and it encoded a 289 amino acid protein. PitPAL was found to have extensive homology with those of other plant PAL genes via multiple alignments. The domination sites and catalytic active sites in PAL protein of Oryza sativa and Zea mays were also found in PitPAL. Phylogenetic tree analysis revealed that PitPAL had closer relationship with PALs from arbor plants than from other plants. The arbor plant is Nerium oleander and Camellia sinensis. PitPAL is a useful tool to study the regulation of anthocyanin metabolism in P. tobira.

关 键 词：PitPAL 苯丙氨酸解氨酶 海桐 基因克隆 序列分析 

分 类 号：Q943.2[生物学—植物学]