H5亚型禽流感病毒HA基因在WISH细胞中的瞬时表达  

Transient Expression of HA Gene of Avian Influenza Virus Subtype H5 in WISH Cell

作　　者：张蕾[1] 张大鹏 吴东林[3] 王诺[1] 明宇[1] 张慧瑛[1] 张瑞兴[1] 张玉静[1] 

机构地区：[1]吉林大学畜牧兽医学院 [2]长春市动物卫生监督所,长春130021 [3]长春市疾病预防控制中心,长春130033

出　　处：《中国农学通报》2008年第3期5-8,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目(C02030604)

摘　　要：在WISH细胞中,对H5亚型禽流感病毒HA基因进行了瞬时表达的研究。首先构建了真核表达质粒pVAX-H5HA,通过酶切和PCR筛选阳性克隆后测序。然后将重组表达质粒以脂质体介导法转染WISH细胞,通过RT-PCR在转录水平检测目的基因的表达,间接免疫荧光检测目的蛋白。结果表明,HA基因在WISH细胞中进行了表达。Transient expression of HA gene of subtype H5 avian influenza virus in WISH cell was detected in this study. First, pVAX-HSHA plasmids were constructed and initially screening by means of PCR and double digestion then further ensured by sequencing. WISH cell was transferred with pVAX-HSHA plasmids by means of lipofectamine media methods. Transient expression were evaluated by RT-PCR and immunofluorescence. The result showed that the HA gene was expressed in WISH cell.

关 键 词：H5亚型禽流感病毒 HA基因 WISH细胞 瞬时表达 

分 类 号：S85[农业科学—兽医学]