Enrichment of putative human epidermal stem cells based on cell size and collagen type IV adhesiveness  

作　　者：Juxue Li Chenglin Miao Welxiang Guo Liwei Jia Jiaxi Zhou Baohua Ma Sha Peng Shuang Liu Yujing Cao Enkui Duan 

机构地区：[1]State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang Dis- trict, Beijing 100101, China [2]Graduate School of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100039, China

出　　处：《Cell Research》2008年第3期360-371,共12页细胞研究（英文版）

摘　　要：The enrichment and identification of human epidermal stem cells （EpSCs） are of paramount importance for both basic research and clinical application. Although several approaches for the enrichment of EpSCs have been established, enriching a pure population of viable EpSCs is still a challenging task. An improved approach is worth developing to enhance the purity and viability of EpSCs. Here we report that cell size combined with collagen type IV adhesiveness can be used in an improved approach to enrich pure and viable human EpSCs. We separated the rap- idly adherent keratinocytes into three populations that range in size from 5-7 μm （population A）, to 7-9 μm （population B）, to ≥9μm （population C） in diameter, and found that human putative EpSCs could be further enriched in population A with the smallest size. Among the three populations, population A displayed the highest density of plintegrin receptor, contained the highest percentage of cells in G0/G1 phase, showed the highest nucleus to cytoplasm ratio, and possessed the highest colony formation efficiency （CFE）. When injected into murine blastocysts, these cells participated in multi-tissue formation. More significantly, compared with a previous approach that sorted putative EpSCs according to pl-integrin antibody staining, the viability of the EpSCs enriched by the improved approach was significantly enhanced. Our results provide a putative strategy for the enrichment of human EpSCs, and encourage further study into the role of cell size in stem cell biology.

关 键 词：epidermal stem cells collagen type Ⅳ cell size Β1-INTEGRIN 

分 类 号：Q2[生物学—细胞生物学]